We narrowed to 17,639 results for: URE

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GST-PRMT9 WT

Plasmid

#67607

Purpose

bacterial expression of wild type human PRMT9

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

wild type

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pHCMV_CHHF_GPC

Plasmid

#207260

Purpose

Mammalian expression of Chapare hemorrhagic fever virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-AsCas12f-2NLS

Plasmid

#171613

Purpose

NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

NLS-AsCas12f1-NLS

Expression

Bacterial

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCyPet-C1

Plasmid

#22779

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

CyPet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
H6-auxilin

Plasmid

#62571

Purpose

6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria

Depositor

Ernst Ungewickell

Article

Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304

Insert

Auxilin

Tags

6XHis

Expression

Bacterial

Mutation

*see comment below

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-Dppa3

Plasmid

#13365

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 3 (Dppa3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pYpet-C1

Plasmid

#22780

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Ypet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
PNMT

Plasmid

#64078

Purpose

Expression of human Phenylethanolamine N-methyltransferase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

Insert

Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only
pHCMV_SLEV_PrME

Plasmid

#207309

Purpose

Mammalian expression of St. Louis encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SLEV_PrME (POLY St. Louis encephalitis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_MVEV_PrME

Plasmid

#207295

Purpose

Mammalian expression of Murray valley encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MVEV_PrME

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-PKG-hNo.7

Plasmid

#175812

Purpose

The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

PKG #7

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNP561

Plasmid

#222723

Purpose

Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLRD16

Plasmid

#208268

Purpose

Production of syn-stemar-13-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL8

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Zfp521 full length

Plasmid

#104189

Purpose

Expression of full length Zfp521 in mammalian cells

Depositor

Kathryn Hentges

Article

AlDallal et al Leuk Res. 2016 Jul;46:10-7. doi: 10.1016

Insert

Zfp521 (Zfp521 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSe-Rp1

Plasmid

#111644

Purpose

For construction of spacer and repeat arrays for Sulfolobus islandicus REY154A

Depositor

Qunxin She

Article

Peng et al Nucleic Acids Res. 2015 Jan;43(1):406-17

Type

Empty backbone

Use

Archaeal expression vector

Promoter

araS

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFEC

Plasmid

#67060

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFEC (TFEC Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBEL1994

Plasmid

#139898

Purpose

Expression plasmid for LetB PLL2 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta229-239, D228G, L240G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2511

Plasmid

#195625

Purpose

Complementation of MlaA(WT)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

mouse CamKII alpha

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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HS-H2B-RFP

Plasmid

#105953

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

H2B

Tags

RFP

Expression

Bacterial

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Article

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-MCH

Plasmid

#184606

Purpose

For expression of a fluorescent sensor for melanin-concentrating hormone in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-MCH

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(Gs)

Plasmid

#230007

Purpose

Expresses anti-GFP PAGER(Gs) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM1Dxs

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pIEx4-Dio-GFP

Plasmid

#198278

Purpose

Cre reporter for insect cells; double-floxxed-EGFP

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pIEx4-Dio-GFP

Expression

Insect

Promoter

IE1 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/Luci

Plasmid

#139981

Purpose

Expresses luciferase under the promoter of CAG. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Luciferase

Use

AAV

Promoter

CAG

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-Rsn2

Plasmid

#61686

Purpose

bacterial expression of ranaspumin 2 as a His-tagged fusion protein

Depositor

Brian Smith

Article

Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

Insert

Ranaspumin-2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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B2395_CIBN-BTB-IDR-FRB

Plasmid

#215107

Purpose

Expresses CIBN-BTB-mCherry-FUS(IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CIBN-BTB-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-A4DARPin

Plasmid

#198288

Purpose

Produces PVCs retargeted with anti-lysozyme DARPin A4

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-A4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)

Plasmid

#229775

Purpose

enhancer AAV for expression of ChrimsonR-tdTomato under the DLX2.0 enhancer

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Matveev et al bioRxiv 2024.11.01.621418

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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