We narrowed to 10,522 results for: SEC
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Plasmid#59050Purposefor baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)DepositorAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only
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pUASP YFP Rab5 DN
Plasmid#37694DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS43N (dominant negative)PromoterGAL4Available SinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_726
Plasmid#176661PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
TagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_763
Plasmid#176658PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab5 CA
Plasmid#37702DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ88L (constitutive active)PromoterGAL4Available SinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
UAS-Xbp1-GFP
Plasmid#80627Purposeexpression of Xbp1-GFP (RA isoform) in DrosophilaDepositorAvailable SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGN33
Plasmid#131105PurposeAn integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutantsDepositorInsertP-furA102tetO-pip
ExpressionBacterialMutationthe furA102 promoter was mutated with the inserti…Available SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
Plasmid#169370PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
UseCRISPRExpressionInsectPromoterCPIJ039596Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab11 WT
Plasmid#37711DepositorAvailable SinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 DN
Plasmid#44826DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationT22N (dominant negative)Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW2
Plasmid#166708Purposeexpresses NEDD4L with inactivating mutation in second WW domain in mammalian cellsDepositorInsertNEDD4L W453A
TagsFLAGExpressionMammalianMutationchanged tryptophan 453 to alaninePromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGW-dpse-GAL4-DBD
Plasmid#125153PurposeGateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CPDepositorInsertD. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
UseGateway fly expression vectorExpressionInsectAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPNprM-hp
Plasmid#48120PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoterDepositorInsertsignal peptide of NprM
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-mTFP
Plasmid#58327PurposeDestination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genomeDepositorInsertCad-mTFP
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-NS1-HilA
Plasmid#153327PurposeTriggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinoseDepositorInsertSptP120-NS1 Monobody
UseSalmonella expressionAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3a pro-hRegIIIa
Plasmid#64935Purposebacterial expression of full length human RegIIIalphaDepositorInsertRegIIIa (REG3A Human)
ExpressionBacterialMutationmutations made in 5' end to relax secondary …PromoterT7Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab6 CA
Plasmid#37698DepositorInsertRab6 (Rab6 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ71L (constitutive active)PromoterGAL4Available SinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab6 DN
Plasmid#37697DepositorInsertRab6 (Rab6 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT26N (dominant negative)PromoterGAL4Available SinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab11 DN
Plasmid#37712DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS25N (dominant negative)PromoterGAL4Available SinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSPVpr-hp
Plasmid#48119PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoterDepositorInsertsignal peptide of Vpr
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
D-raf R217L
Plasmid#39762DepositorAvailable SinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rae1-pDONR221
Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
ExpressionInsectAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC 638–1457
Plasmid#59051Purposefor baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)DepositorAvailable SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC 776–1457
Plasmid#59052Purposefor baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)DepositorAvailable SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab7 CA
Plasmid#37704DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC 3GLA UAS HAi
Plasmid#71763Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tagDepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
ReaLiSM A1-Hinge-GPIba+2cys
Plasmid#59581Purposeexpress VWF A1-GPIba ReaLiSM construct in mammalian cellsDepositorAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOT2 3GLA
Plasmid#71764Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistantDepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pUASp YFP Rab11 CA
Plasmid#53472DepositorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMCh-139
Plasmid#92287PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cellsDepositorAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5A7-T
Plasmid#92020PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N and C-terminal TEV protease cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab6 DN
Plasmid#58448Purposeexpression of drosophila YFP-Rab6 dominant negative mutantDepositorAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab6 WT
Plasmid#58447Purposeexpression of drosophila YFP-Rab6DepositorAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-VHL-Flag(117-213)
Plasmid#29501DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab1 DN
Plasmid#37682DepositorInsertRab1 (Rab1 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS25N (dominant negative)PromoterGAL4Available SinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
D10 pAcGP67a
Plasmid#41812DepositorAvailable SinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab7 DN
Plasmid#37703DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only