We narrowed to 149,174 results for: ins;

1. pDONR221-SLC27A1_STOP

   Plasmid

   #161294

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC27A1 (SLC27A1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDONR221-SLC44A1_STOP

   Plasmid

   #161079

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC44A1 (SLC44A1 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pDONR221-SLC27A5_STOP

   Plasmid

   #161270

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC27A5 (SLC27A5 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

   Plasmid

   #197505

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

   Promoter

   CMV and T7

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169164

   Purpose

   Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
   

   Tags

   6His-3xFlag (nsp10 C-terminus)

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR221-LETM1_STOP

   Plasmid

   #161456

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   LETM1 (LETM1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDONR221-SLC25A16_STOP

   Plasmid

   #161145

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A16 (SLC25A16 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pDONR221-MFSD11_STOP

   Plasmid

   #161094

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   MFSD11 (MFSD11 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDONR221-SLC35E2A_STOP

   Plasmid

   #161252

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35E2A (SLC35E2 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pDONR221-SLCO1C1_STOP

    Plasmid

    #161390

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO1C1 (SLCO1C1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pDONR221-SLC43A3_STOP

    Plasmid

    #161368

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC43A3 (SLC43A3 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75243

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid

    #169181

    Purpose

    Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    No purification tag and nsp7-nsp8 fusion protein …

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. px330-Apob-2

    Plasmid

    #162549

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. px330-Apob-1

    Plasmid

    #162548

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px330-Apob-3

    Plasmid

    #162550

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px330-Apob-4

    Plasmid

    #162551

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CLV1 L2_pECIA2

    Plasmid

    #115100

    Purpose

    Bait vector CLV1 L2_pECIA2 should be used with prey vector CLV1 L2_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G75820 (CLV1 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GSO2/EDA23 X079_pECIA2

    Plasmid

    #115111

    Purpose

    Bait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G44700 (GSO2 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SERK1 A6_pECIA2

    Plasmid

    #114967

    Purpose

    Bait vector SERK1 A6_pECIA2 should be used with prey vector SERK1 A6_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G71830 (SERK1 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CLV1 L2_pECIA14

    Plasmid

    #114900

    Purpose

    Prey vector CLV1 L2_pECIA14 should be used with bait vector CLV1 L2_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G75820 (CLV1 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SERK3/BAK1 BAKNcFus_pECIA14

    Plasmid

    #114775

    Purpose

    Prey vector SERK3/BAK1 BAKNcFus_pECIA14 should be used with bait vector SERK3/BAK1 BAKNcFus_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT4G33430 (BAK1 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SERK1 A6_pECIA14

    Plasmid

    #114767

    Purpose

    Prey vector SERK1 A6_pECIA14 should be used with bait vector SERK1 A6_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G71830 (SERK1 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MK01_HUMAN_D0

    Plasmid

    #79713

    Purpose

    This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK01 (MAPK1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

25. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75242

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Rb379-928/C706F-pGex2TK

    Plasmid

    #21750

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    Rb (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928 of wt Rb …

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PAX3::FOXO1ΔHD-2A-sfGFP

    Plasmid

    #240099

    Purpose

    human PAX3::FOXO1 with homeobox domain deletion in -2A-sfGFP backbone (Plasmid# 240099)

    Depositor

    Genevieve Kendall

    Article

    Kucinski et al Cell Rep. 2025 Jul 22;44(7):115923. doi:

    Insert

    PAX3::FOXO1-minusHD (FOXO1, PAX3 Human)
    

    Expression

    Mammalian

    Mutation

    removed amino acids 218-278 for the homeobox DNA …

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-mEtfdh-AAA6

    Plasmid

    #232317

    Purpose

    Expression of mouse mutant Etfdh (AAA6)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    Etfdh (Etfdh Mouse)
    

    Use

    Lentiviral

    Mutation

    PAM Sequence Mutations: (G36C; T39C, C42T; C72T, …

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-mEtfdh-AAG6

    Plasmid

    #232315

    Purpose

    Expression of mouse mutant Etfdh (AAG6)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    Etfdh (Etfdh Mouse)
    

    Use

    Lentiviral

    Mutation

    PAM Sequence Mutations: (G36C, T39C, C42T, C72T, …

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 7AA-aSyn-pRK172

    Plasmid

    #236197

    Purpose

    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.

    Depositor

    Sjors Scheres, Yang Yang

    Article

    Yang et al Acta Neuropathol. 2023 May;145(5):561-57

    Insert

    SNCA (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    insertion os MAAAEKT at residue 22

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-SpCas9-P2A-EGFP (MSP2582)

    Plasmid

    #223067

    Purpose

    pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTR01F/hFGFR3b-V5

    Plasmid

    #236016

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR3b protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR3b (FGFR3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    truncated CMV promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTR01F/hFGFR4-V5

    Plasmid

    #236017

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR4 protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR4 (FGFR4 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    truncated CMV promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-mEtfdh-AAA4

    Plasmid

    #232316

    Purpose

    Expression of mouse mutant Etfdh (AAA4)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    Etfdh (Etfdh Mouse)
    

    Use

    Lentiviral

    Mutation

    PAM Sequence Mutations: (G36C; T39C, C42T; C72T, …

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TH1150-pOCC177-FUS(Nhe)-PLD_YtoF_RGG_RtoK

    Plasmid

    #221889

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    In PLD all Tyr (Y) changed to Phe (F), in RGG (aa…

    Promoter

    PH promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMVtrunc LifeactΔC4-msfGFPΔN7

    Plasmid

    #231555

    Purpose

    Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Lifeact (ABP140 Budding Yeast)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1-FRB:GSlinker:SSD

    Plasmid

    #228535

    Purpose

    FRB protein in fusion with a bipartite SSD degron, consisting of fragments of human Skp1 and SOCS2 proteins, through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    FRB:GSlinker:SSD (SKP1, SOCS2 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCR8/GW_TOPO_Cdk13_Untagged

    Plasmid

    #127342

    Purpose

    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    None

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid

    #217803

    Purpose

    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Unfused click editor construct; mSA-ePhi29 - pCMV-T7-mSA-Phi29 (JO1411)

    Plasmid

    #217807

    Purpose

    Unfused click editor construct expressing mSA-ePhi29(D169A), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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