We narrowed to 7,281 results for: 11

1. p026 pCS2-EnR

   Plasmid

   #11028

   Depositor

   Ramesh Shivdasani

   Article

   Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

   Insert

   engrailed (en Fly)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Mutation

   Engrailed repressor domain (bases encoding amino …

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-Puro-REDD1-HA

   Plasmid

   #133550

   Purpose

   Expresses HA tagged REDD1 in Mammalian cells

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HypaCas9

   Plasmid

   #138557

   Purpose

   Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   HypaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFN18A-HaloTag-Biotin

   Plasmid

   #206039

   Purpose

   HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Insert

   Talin R3-IVVI (Tln1 Mouse)
   

   Tags

   10 x His-AviTag and Halo-Tag

   Expression

   Bacterial

   Mutation

   Threonine 809 to Isoleucine, Threonine 833 to Val…

   Promoter

   T7

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

   Plasmid

   #174896

   Purpose

   Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GAL-p300-1000-1600

   Plasmid

   #89101

   Purpose

   mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 1000-1600

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GAL-p300-2-300

   Plasmid

   #89098

   Purpose

   mammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 2-300

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hARdelta120-472

   Plasmid

   #89088

   Purpose

   mammalian expression of human androgen receptor with deletion of 120-472 amino acid residues

   Depositor

   Elizabeth Wilson

   Article

   Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

   Insert

   hAR-120-472 N-term deletion (AR Human)
   

   Expression

   Mammalian

   Mutation

   hAR-120-472

   Promoter

   CMV

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GAL-p300-1901-2414

   Plasmid

   #89103

   Purpose

   mammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 1901-2414

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-EMTB-TurboID-V5

    Plasmid

    #190736

    Purpose

    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    ensconsin (MAP7 Human)
    

    Tags

    Myc, TurboID, and V5

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-ECFP

    Plasmid

    #112220

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    ECFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK0_062

    Plasmid

    #123951

    Purpose

    Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR DEST
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQHA-USP7 WT puroR

    Plasmid

    #46753

    Purpose

    Retroviral vector that expresses wild type HA-tagged human USP7

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQHA-USP11 WT puroR

    Plasmid

    #46749

    Purpose

    Retroviral vector that expresses wild type HA-tagged wild type human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. ESR1-luciferase

    Plasmid

    #113351

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ESR1-enhancer (ESR1 Human)
    

    Use

    Luciferase

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid

    #140021

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

    Plasmid

    #140018

    Purpose

    Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoTyrRS
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y37L, D182S, F183M, L186A

    Promoter

    EF1

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQHA-USP11 CS puroR

    Plasmid

    #46750

    Purpose

    Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-MyosinVa-Ctail

    Plasmid

    #110169

    Purpose

    Dominant negative effect by competing with MyosinVa C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Va (Myo5a Mouse)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    amino acids 1444-1853

    Promoter

    CMV

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cx30-msfGFP

    Plasmid

    #69019

    Purpose

    Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx30 (GJB6 Human)
    

    Tags

    V206K monomerized super folder GFP

    Expression

    Mammalian

    Promoter

    c

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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