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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,439 results for: eng

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Showing: 3921 - 3940 of 14439 results

  6. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid
    #98975
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 4 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98975-plasmid-map-sequence-id-199218

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  7. EKARdual

    Plasmid
    #99870
    Purpose
    Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate
    Depositor
    Marc Tramier
    Publication
    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EKAR
    Tags
    ShadowG and mTFP1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    99870-plasmid-map-sequence-id-182629

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  9. PX394 Neisseria cinerea Cas9

    Plasmid
    #68331
    Purpose
    Neisseria cinerea Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68331-plasmid-map-sequence-id-119554

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  12. 835 pcDNA3 HA PTEN G129R

    Plasmid
    #10929
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G129R
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  14. MMLV-RT for Split-PE (pEH45)

    Plasmid
    #190105
    Purpose
    Expresses MMLV-RT (full-length, from PE2) for Split-PE use
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLV-RT
    Tags
    bpNLS
    Expression
    Mammalian
    Mutation
    RT pentamutant from PE2 (D200N, T306K, W313F, T33…
    Promoter
    CMV
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190105-plasmid-map-sequence-id-370600

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  20. FUT8-Dox

    Plasmid
    #124631
    Purpose
    Doxycycline-inducible FUT8 expression in mammalian cells
    Depositor
    Ron Weiss
    Publication
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Alpha-(1,6)-fucosyltransferase
    Tetracycline repressor A3 mutant
    TagFP635
    Expression
    Mammalian
    Promoter
    TRE-tight and hEF1a
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124631-plasmid-map-sequence-id-248079

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Showing: 3921 - 3940 of 14439 results