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4930 TRV2-eTnpBc2xSV40nls-HHreRNA4PDS

Plasmid

#251823

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

Enhanced variant of ISDra2 eTnpBc with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

2xSV40nls

Expression

Plant

Mutation

N4Y/R110K/V192L/L222I

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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TFORF2509

Plasmid

#144387

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EP300 (EP300 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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FRB-TagRFPT-CAAX

Plasmid

#87710

Purpose

FRB-tagged TagRFPT; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FRB-TagRFPT-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CyTERM-mGFP

Plasmid

#62237

Purpose

ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.

Depositor

Erik Snapp

Article

Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1

Insert

CyTERM-mGFP

Tags

mGFP

Expression

Mammalian

Mutation

signal anchor of rabbit cytochrome p450 and monom…

Promoter

CMV

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSW071

Plasmid

#140037

Purpose

PCC 11901 fadD locus KO with a kanamycin resistance cassette

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Kanamycin resistance gene

Use

Gene knockout in cyanobacteria

Promoter

KanR

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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Gal4-PGC1 alpha

Plasmid

#8892

Depositor

Bruce Spiegelman

Article

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only
pMXs-Dnmt3l

Plasmid

#13356

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pT7-7 asyn E46K

Plasmid

#105728

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein E46K (SNCA Human)

Expression

Bacterial

Mutation

E46K

Promoter

T7

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-MVPH

Plasmid

#235600

Purpose

Expresses the MS2-VP64-P65-HSF1 fusion protein

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

MS2-VP64-P65-HSF1

Use

Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-Qa1 SCT-T2A-GFP

Plasmid

#205453

Purpose

lentiviral plasmid for expression of mouse Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

Qa1 SCT (H2-T23 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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HDR-HaloPOLQ

Plasmid

#211637

Purpose

halo donor

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

Puro-LoxHaloTag

Tags

flag

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact

Plasmid

#109545

Purpose

Multisite gateway vector for 5' tagging with the actin binding motif Lifeact

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actin binding motif Lifeact

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG35

Plasmid

#35123

Depositor

John McCusker

Article

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

natMX3

Use

Yeast replicating

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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L2_lacZgRNA-Cas9-CsA

Plasmid

#136138

Purpose

Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mNeonGreen-HIS3MX6

Plasmid

#129100

Purpose

For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

Depositor

Wei-Lih Lee

Insert

mNeonGreen

Expression

Yeast

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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R777-E229 Hs.RASA2

Plasmid

#70513

Purpose

Gateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RASA2 (RASA2 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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FAB(PX-LD)

Plasmid

#214426

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FKBP-V5

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-hYTHDC1

Plasmid

#85167

Purpose

Expression of FLAG-HA-tagged human YTHDC1

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDC1 (YTHDC1 Human)

Tags

Flag and Ha

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-FLEx-TetOn3G-3xHA-FLEx

Plasmid

#133729

Purpose

Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G-3xHA (floxed & inverted)

Use

AAV

Tags

3xHA-tag

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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G-CaMP3

Plasmid

#22692

Purpose

a single-wavelength GCaMP2-based genetically encoded calcium indicator

Depositor

Loren Looger

Article

Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Insert

G-CaMP3

Expression

Mammalian

Mutation

N363D mutation (increased fluorescence)

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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