We narrowed to 5,123 results for: puc

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pMGS59(AID-ARF-P2A-Hygromycin)

Plasmid

#138174

Purpose

An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

Depositor

Michael Guertin

Article

Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

Insert

AID-ARF-P2A-Hygromycin

Tags

AID-ARF clamp degron tag

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Cquin_596

Plasmid

#176655

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pVH617

Plasmid

#169606

Purpose

Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4 / PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL704

Plasmid

#140374

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-4BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pPN217

Plasmid

#91676

Purpose

Express sgRNA targeting human TLE1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TLE1 (TLE1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMG

Plasmid

#138264

Purpose

Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Inserts

eGFP flanked by iCas9 sites
mCherry

Use

CRISPR

Expression

Yeast

Promoter

Tef1

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL705

Plasmid

#140375

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-5BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAT15485_Hypa-ABE8e

Plasmid

#174123

Purpose

Expresses ABE8e fused with Hypa-SpCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-ABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Empty Anchor Control

Plasmid

#127665

Purpose

Encodes for puromycin resistance expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

Puro

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTL-Bag3-gRNA3

Plasmid

#196254

Purpose

Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag2-gRNA2

Plasmid

#196252

Purpose

Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag1-gRNA1

Plasmid

#196251

Purpose

Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag4-gRNA4

Plasmid

#196255

Purpose

Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag2T-gRNA2

Plasmid

#196253

Purpose

Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBCV

Plasmid

#62659

Purpose

Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FLPo

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pDT-sgRNA-XMAS-2x

Plasmid

#164414

Purpose

Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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TC1464

Plasmid

#164881

Purpose

CMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

dcas13d-ADARdd

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE663

Plasmid

#153261

Purpose

M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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