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pTK312

Plasmid

#59569

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP XbaI attB1 Kozak EGFP ochre attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pIF1079

Plasmid

#237445

Purpose

Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

Depositor

IIya Finkelstein

Article

Buffington et al Nat Biotechnol. 2025 Oct 23. doi: 10.103

Inserts

SpCas9
Efe1-RT
EMX1 sgRNA
Efe1-ncRNA targeting EMX1

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG, H1, and U6

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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JD690

Plasmid

#160397

Purpose

Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::citrus GRF4-GIF1 chimera

Promoter

35S

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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VRQR-ABEmax

Plasmid

#119811

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GlyRS-His

Plasmid

#124110

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[TEV]

Plasmid

#200122

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Myosin-IIA-GFP

Plasmid

#38297

Depositor

Matthew Krummel

Article

Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

Insert

Myosin II A (Myh9 Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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Circular guide cloning vector

Plasmid

#180184

Purpose

Cloning vector for expressing circular RNA from a U6 promoter

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-miniTurbo-V5_pCDNA3

Plasmid

#107174

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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PrimeRoot.v2C-Cre

Plasmid

#201739

Purpose

For DNA insertion test in protoplast

Depositor

Caixia Gao

Article

Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

ePPE,Cre

Use

CRISPR

Expression

Plant

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pHSB04X

Plasmid

#117258

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019

Use

Other

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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p131CB10-tphKAB

Plasmid

#241911

Purpose

TPA catabolic operon under the control of a PCA inducible biosensor

Depositor

Neil Dixon

Article

Chacon et al Trends Biotechnol. 2025 Mar;43(3):647-67

Insert

tphK, tphA2, tphA3, tphB, tphA1

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLM-fSV2A

Plasmid

#27512

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

OCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2

Use

Lentiviral

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pSL1119 (pDonor)

Plasmid

#160732

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA (Mini-Tn)

Expression

Bacterial

Promoter

N/A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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PrimeRoot.v2C-FLP

Plasmid

#201740

Purpose

For DNA insertion test in protoplast

Depositor

Caixia Gao

Article

Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

ePPE,FLP

Use

CRISPR

Expression

Plant

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA_112

Plasmid

#136475

Purpose

Lentiviral expression of AsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

AsCas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre

Plasmid

#243748

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20-Pdp1_NTD-Cre

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

Plasmid

#225432

Purpose

Transcriptional unit of BeYDV Rep/RepA (C1:C2)

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

pNOS:Rep/RepA:tNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9

Plasmid

#243744

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_HUHe-Pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro Xlone-eGFP

Plasmid

#136936

Purpose

donor plasmid for inducible transgene expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

all-in-one tet-on system

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a2

Plasmid

#177874

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized FnCas12a

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACT4)

Plasmid

#235060

Purpose

Expresses Tad-CBE(ACT4) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACT4)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor-LoxP

Plasmid

#219436

Purpose

CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

two transposon ends, LoxP-terminator-LoxP gene cassette

Expression

Bacterial

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDY1243-Fluorescent RADARS

Plasmid

#182540

Purpose

Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

mNeon

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGS-SAP30

Plasmid

#109212

Purpose

Encodes human full-length SAP30 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

Human full-length SAP30 (SAP30 Human)

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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