We narrowed to 54,283 results for: Ran

1. shRNA-Ratio-PosCon

   Plasmid

   #183553

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio positive control (Pbrm1), Figure S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Pbrm1 shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. shRNA-Ratio-NegCon

   Plasmid

   #183554

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Luciferase shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKDR-TetOn.13

   Plasmid

   #183584

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 13
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKDR-EF1a.10

   Plasmid

   #183573

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKDR-EF1a.11

   Plasmid

   #183574

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKDR-EF1a.12

   Plasmid

   #183575

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 12
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKDR-EF1a.5

   Plasmid

   #183572

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKDR-EF1a.3

   Plasmid

   #183570

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDR-EF1a.1

   Plasmid

   #183568

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKDR-CBh.21

    Plasmid

    #183565

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Attenuator Sequence 21
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1

    Plasmid

    #182376

    Purpose

    Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    3xFlag
    mito-mTFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter and SV40 promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0CM0058

    Plasmid

    #177022

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol 8-oxidase; geraniol-10-hydroxylase; CYP76B6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD0CM0059

    Plasmid

    #177023

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0CM0063

    Plasmid

    #177021

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    transit peptide removed

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJo55

    Plasmid

    #159623

    Purpose

    An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.

    Depositor

    Vasiliy Taranov

    Article

    Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-HA-PGRMC1-1-54

    Plasmid

    #173472

    Purpose

    PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1 (PGRMC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted for amino acids 55-194

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-HA-PGRMC1-21-195

    Plasmid

    #173473

    Purpose

    PGRMC1 lacking the N-terminal region upstream of the transmembrane region

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1 (PGRMC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted for amino acids 1-20

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAraQCas-BsaI

    Plasmid

    #170633

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJCC_084 Cas12i1 D647A

    Plasmid

    #171671

    Purpose

    For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1 D647A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 647 to alanine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

    Plasmid

    #162805

    Purpose

    STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI-HA_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBEL2082

    Plasmid

    #155162

    Purpose

    Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    (MlaFΔ[248-269])-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 248-269 in MlaF

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBEL1514

    Plasmid

    #155147

    Purpose

    Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBEL1965

    Plasmid

    #155152

    Purpose

    Expresses MlaF(1-246)-(6 aa linker)-GCN4

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBEL1966

    Plasmid

    #155153

    Purpose

    Expresses MlaF(1-246)-(10 aa linker)-GCN4

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBEL1967

    Plasmid

    #155154

    Purpose

    Expresses MlaF(1-246)-(14 aa linker)-GCN4

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGST-POLA1-N0

    Plasmid

    #160983

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGST-POLA1-N1-2A

    Plasmid

    #160986

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    W84A/D88A

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGST-POLA1-N2-2A

    Plasmid

    #160987

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    W84A/D88A

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGST-POLA1-N2

    Plasmid

    #160985

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge