We narrowed to 37,484 results for: Ran;

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro cPLA2-EGFP

Plasmid

#162568

Purpose

Used as a measure of cPLA2 activation in live cells with an EGFP tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTK88_GFP-Anillin

Plasmid

#46354

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

Anillin (ANLN Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

a1854 is changed to c to disrupt XhoI site withou…

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pRG5273Neo

Plasmid

#26388

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pMaster3

Plasmid

#58526

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 )
myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
Nanog (NANOG Human)
IRES
NR5A2 (NR5A2 Human)

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-TfR

Plasmid

#12130

Depositor

Pamela Bjorkman

Article

Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

Insert

transferrin receptor (TFRC Human)

Tags

6X-His tag, Factor Xa cleavage site, and leader p…

Expression

Insect

Mutation

The portion of the human TfR gene encoding residu…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pYM70

Plasmid

#119922

Purpose

CaHygB plasmids that facilitate genetic manipulations in the fungal pathogen

Depositor

Brian Wong

Article

Basso LR et al Yeast. 2010 Dec;27(12):1039-48. doi: 10.

Insert

CaHygB

Tags

CaHygB

Promoter

TEF2

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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T7_CC_PE7_IVT_Template

Plasmid

#223022

Purpose

Template for in vitro transcription of PE7. For HSPC-related experiments.

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_6xH

Plasmid

#186461

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-SialT

Plasmid

#87324

Purpose

To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase

Depositor

Lei Lu

Article

Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

Insert

ST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

Plasmid

#58959

Purpose

lentiviral expression of v-H-Ras and luciferase/EGFP reporter

Depositor

Snorri Thorgeirsson

Article

Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

Inserts

CMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene

Use

Lentiviral

Expression

Mammalian

Promoter

CMV enhancer/chicken beta actin

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-NLS-mEGFP

Plasmid

#105527

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Nucleoplasmin

Use

In vitro transcription

Tags

mEGFP

Mutation

NLS of nucleoplasmin (encoding amino acids KRPAAT…

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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Rab8-T22N(Flag) in pcDNA3.1neo

Plasmid

#46784

Purpose

expression of T22N Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A T22N (RAB8A Human)

Tags

FLAG

Expression

Mammalian

Mutation

T22N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
R26P-M2rtTA

Plasmid

#47381

Purpose

Rosa 26 targeting vector

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Inserts

M2rtTA
pgk-puro

Use

Mouse Targeting

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
pAA173

Plasmid

#21743

Depositor

David Sherwood

Article

Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

Insert

pie-1>mCherry::PH domain (PLC-delta)

Use

Bacterial propagation

Tags

mCherry

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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PBCAG-EGFR

Plasmid

#40974

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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LV-TRE-WT mouse MyoD-T2A-dsRedExpress2

Plasmid

#60624

Purpose

Expresses WT mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

WT mouse MyoD (Myod1 Mouse)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pIND-STAT1-V5

Plasmid

#11618

Depositor

Steven Johnson

Insert

Signal transducer and activator of transcription 1 (STAT1 Human)

Use

Ecdysone inducible

Tags

V5

Expression

Mammalian

Mutation

no mutations

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only