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pFRT/FLAG/HA-DEST EIF2C1

Plasmid

#19887

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 1 (AGO1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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rAAV9-CAP-in-cis-Lox-AAV

Plasmid

#205990

Purpose

rAAV9 cap acceptor vector for CREATE libraries

Depositor

Viviana Gradinaru

Article

Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

Insert

rAAV9 cap acceptor vector for CREATE libraries

Use

AAV

Mutation

Deletion between XbaI and AgeI sites

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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mCol.4F2A targeting construct

Plasmid

#25794

Depositor

Rudolf Jaenisch

Article

Carey et al Nat Methods. 2010 Jan . 7(1):56-9.

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-3sgRNA

Plasmid

#64241

Purpose

destination vector for three U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xPCP-2xGFP

Plasmid

#71243

Purpose

Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

2xPCP-2xGFP

Use

P element-mediated germline transformation

Tags

EGFP

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2

Plasmid

#58681

Purpose

Expresses 3x Flag-tagged LGP2 in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

laboratory of genetics and physiology 2 (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-Keap1 delta BTB

Plasmid

#21593

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

kelch-like ECH-associated protein 1 (KEAP1 Human)

Tags

HA2

Expression

Mammalian

Mutation

Delta BTB (N178) deletion

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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VPR-ps-dSpCas9

Plasmid

#102855

Purpose

A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

dSpCas9, pdDronpa1.2

Use

CRISPR and Synthetic Biology

Tags

NLS and VP64-p64-Rta transcription activator

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE4

Plasmid

#197148

Purpose

Expresses ApoE4 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE4 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDMS197

Plasmid

#43831

Depositor

Dieter Schifferli

Article

Edwards et al Gene. 1998 Jan 30;207(2):149-57.

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb1

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCM184-ParB2::eGFP

Plasmid

#87252

Purpose

Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB2

Tags

EGFP

Expression

Yeast

Promoter

pBAD/tetO

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-MED1long-IDR-YFP-NLS

Plasmid

#145276

Purpose

Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MED1-IDR long (MED1 Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-mCherry

Plasmid

#65265

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphai1-RLuc8

Plasmid

#140973

Purpose

Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphai1-RLuc8 (GNAI1 Human)

Use

Synthetic Biology

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4-AARE-luc2P-Hygro

Plasmid

#101787

Purpose

Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

3xAARE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Promoter

NF-kappaB binding site

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Sox10

Plasmid

#46536

Purpose

A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Sox10 (Sox10 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-p65AD-GBP6

Plasmid

#49440

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-p65 transactivation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Rabenosyn-5

Plasmid

#37538

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

Rabenosyn5 (RBSN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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