We narrowed to 58,407 results for: tra

1. pAAV-CaMKII-GAL4-iLight-VP16

   Plasmid

   #170274

   Purpose

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
   

   Use

   AAV

   Mutation

   See Comments

   Promoter

   CaMKII

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSB117 - pL2_pSB90_fLUC-I_V2

   Plasmid

   #123192

   Purpose

   binary plant vector for transient TYLCV V2 and fLUC (with intron) expression

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::V2::tNOS 2x35S::fLUC-I::tNOS
   

   Use

   Luciferase

   Expression

   Plant

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEA01

   Plasmid

   #170766

   Purpose

   Utilisation of pSAM2 excision tool in Amycolatopsis spp

   Depositor

   Jean-Luc Pernodet

   Article

   Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

   Inserts

   xis, int
   oriT
   pac
   

   Expression

   Bacterial

   Promoter

   trcp

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV4Neo-murine IL-17RC(FL)

   Plasmid

   #46864

   Purpose

   expresses mouse IL-17RC (all exons)

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RC (Il17rc Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTwist-SARS-CoV-2 Δ18 P.1

   Plasmid

   #173476

   Purpose

   Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 Spike Truncated to remove 18 amino acids
   

   Expression

   Mammalian

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGG-9.47_VP1,3 #2515

   Plasmid

   #74237

   Purpose

   Modified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47 VP1
   AAV9.47 VP3
   

   Use

   AAV

   Available Since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV CytoBAS

   Plasmid

   #62860

   Purpose

   Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

   Depositor

   Stan van de Graaf

   Article

   van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

   Insert

   CytoBAS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG876 pDisplay-pFAST

   Plasmid

   #172868

   Purpose

   expresses pFAST at the surface of mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   pFAST-PDGFR
   

   Tags

   IgK leader sequence (N terminal on insert) and My…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGG-9.47_VP2_AS #2562

   Plasmid

   #74238

   Purpose

   Modified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47 VP2-AS
   

   Use

   AAV

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV NucleoBAS

    Plasmid

    #62861

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSF4 CMV 5TOP intron renilla TRICK CTE polyA

    Plasmid

    #64544

    Purpose

    5′ TOP TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    12x PP7 stem-loops
    

    Tags

    24xMS2 stem loops, 5' TOP (5′ terminal oligo…

    Expression

    Mammalian

    Promoter

    Tetracycline-inducible promoter and 5′ UTR of hum…

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF0706

    Plasmid

    #143432

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NCOR1 (NCOR1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NKP90 RFP (787)

    Plasmid

    #62001

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    NKP90 (TMEM126A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    pgk

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

    Plasmid

    #176542

    Purpose

    Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Tags

    3' extension derived from endogenous locus a…

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TfR-moxGFP-DHFR

    Plasmid

    #173012

    Purpose

    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    TfR-moxGFP-DHFR
    

    Tags

    DHFR and moxGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV/myc/ER-αT9ib

    Plasmid

    #110768

    Purpose

    Expresses ER intrabody against mouse and human TLR9

    Depositor

    Thomas Böldicke

    Article

    Reimer et al Cell Mol Biol Lett. 2013 Sep;18(3):433-4

    Insert

    intrabody against mouse and human TLR9
    

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

    Plasmid

    #176538

    Purpose

    Mammalian expression of nuclear localized AwaIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    AwaIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB116 - pL2_pSB90_fLUC-I_P19

    Plasmid

    #123191

    Purpose

    binary plant vector for transient TBSV P19 and fLUC (with intron) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::P19::tNOS 2x35S::fLUC-I::tNOS
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB118 - pL2_pSB90_fLUC-I_HcPro

    Plasmid

    #123193

    Purpose

    binary plant vector for transient TEV HcPro and fLUC (with intron) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTwist-WIV1-CoV

    Plasmid

    #164438

    Purpose

    Encodes WIV1-CoV Spike Protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    WIV1-CoV Spike
    

    Expression

    Mammalian

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge