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8,917 results

Showing: 3961 - 3980 of 8917 results

  2. pET-scFv-T

    Plasmid
    #67843
    Purpose
    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.
    Depositor
    Joanna Bereta
    Article
    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24
    Insert
    antibody 1D8 light kappa chain
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67843-plasmid-map-sequence-id-117725

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  3. Lenti-AsCpf1-Blast

    Plasmid
    #84750
    Purpose
    Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    AsCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    84750-plasmid-map-sequence-id-164466

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  6. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  11. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Insert
    Villin-mTQ2-P2A-FlpO-ERT2
    Tags
    mTQ2, linked to FlpO through an P2A ribosomal ski…
    Expression
    Mammalian
    Promoter
    Villin
    Available Since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    67278-plasmid-map-sequence-id-115887

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  12. mCherry-Gamma-Tubulin-17

    Plasmid
    #55050
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Gamma-Tubulin (TUBG1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55050-plasmid-map-sequence-id-215600

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  15. mCherry-H3-23

    Plasmid
    #55058
    Purpose
    Localization: Nucleus/Histones, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    H3 (H3C2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55058-plasmid-map-sequence-id-424732

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  19. pUCmini-iCAP-AAV.CAP-B22

    Plasmid
    #175005
    Purpose
    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    Use
    AAV
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available Since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175005-plasmid-map-sequence-id-347133

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  20. pAAV-Ef1a-DIO EYFP

    Plasmid
    #27056
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    EYFP
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27056-plasmid-map-sequence-id-126562

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Showing: 3961 - 3980 of 8917 results