We narrowed to 11,499 results for: aga

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pTorPE-G-GECO1.2

Plasmid

#32468

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/TO-myc-BirA*-NES

Plasmid

#112714

Purpose

For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

HIV Rev nuclear export signal (NES)

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-STAG2 shRNA 1221

Plasmid

#31978

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

STAG2 (STAG2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEE491

Plasmid

#149292

Purpose

T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCK081 desmaMCS:minpromCerulean,exorh:mCherry

Plasmid

#200014

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHis2-Arf1 (17-181)-Q71L

Plasmid

#184808

Purpose

Bacterial expression of Arf1 (aa17-181, Q71L mutation)

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Arf1 (ARF1 Human)

Tags

His6

Expression

Bacterial

Mutation

aa 17-181 only, Q71L

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVk7-33G1-Flag

Plasmid

#58950

Purpose

backbone for mouse recombinant antibody with added C-terminal FLAG tag

Depositor

Gavin Wright

Article

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAd-Sox2

Plasmid

#19767

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Science. 2008 Nov 7;322(5903):945-9. doi

Insert

Sox2 (Sox2 Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only
pClneoMyc human MOB1

Plasmid

#37024

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

MOB1B (MOB1B Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
pMK282 (mCherry2-Bsr)

Plasmid

#72798

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 141-465

Plasmid

#45971

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 141-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgPREX1

Plasmid

#86127

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

gRNA targeting PREX1 (PREX1 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEE531

Plasmid

#149290

Purpose

T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR_hEMSY

Plasmid

#125156

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

EMSY (EMSY Human)

Use

Gateway Cloning

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-puro human lipin 1-1 shRNA

Plasmid

#32019

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

Plasmid

#162486

Purpose

For generating transgenic mice expressing ERK biosensor and RGECO1

Depositor

Michiyuki Matsuda

Article

Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

Insert

ERK biosensor based on FRET, and RGECO1

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFLAG-ING5

Plasmid

#13292

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING5 (ING5 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pSH1/Mf-del-Akt-E

Plasmid

#15288

Depositor

David Spencer

Article

Park et al Nat Biotechnol. 2006 Dec . 24(12):1581-9

Insert

delPH-Akt (Akt1 Mouse)

Tags

HA epitope and Myristoylation-targeting domain Fy…

Expression

Mammalian

Mutation

Truncated PH (residues 1-99)

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pTorPE-IP2.0

Plasmid

#109217

Purpose

Express IP2.0 in E. coli

Depositor

Takeshi Ishihara

Article

Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

Insert

IP2.0 (Inverse Pericam 2.0)

Tags

His-tag

Expression

Bacterial

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSb_init containing the TM287/288 nanobody Nb_TM#2

Plasmid

#132698

Purpose

expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

nanobody Nb_TM#2

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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