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Plasmid#169927PurposeWT U6 with a U57A mutationDepositorInsertU6 U57A
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2
Plasmid#209061PurposeEntry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.DepositorInsertKDM6A
UseGateway vector to be used for lr reactionTagsExpressionMutationPromoterU6Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-CMV-Cre-U6-sgNotch2#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2
Plasmid#209069PurposeEntry vector that encodes sgRNAs against mouse Notch2, Rb1, Trp53, Rbl2, and CMV Cre recombinase.DepositorInsertsgRNAs targeting Notch2, Rb1, Trp53, Rbl2 and Cre recombinase
UseGateway vector to be used for lr reactionTagsExpressionMutationPromoterU6Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-CMV-Cre-U6-sgNotch1#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2
Plasmid#209068PurposeEntry vector that encodes sgRNAs against mouse Notch1, Rb1, Trp53, Rbl2, and CMV Cre recombinase.DepositorInsertsgRNAs targeting Notch1, Rb1, Trp53, Rbl2 and Cre recombinase
UseGateway vector to be used for lr reactionTagsExpressionMutationPromoterU6Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6::sgBFP-U6::sgKrasG12D-pX333-red
Plasmid#149448PurposepX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.DepositorInsertsgRNA BFP and mouse KRAS G12D
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo U6-SpAi9R-U6-SpDMDR
Plasmid#78609PurposeExpresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23DepositorInsertU6-SpAi9R-U6-SpDMDR
UseUnspecifiedTagsExpressionMutationPromoterU6Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-pegSERPINA1-U6-Nicking-PE2-N
Plasmid#164907PurposeExpress a pegRNA targeting SERPINA1, and a nicking sgRNA and the N-terminal split-intein fragment of PE2DepositorInsertpegSERPINA1, Nicking sgRNA, PE2-N
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD4d-U6-1:white1-U6-3:white1
Plasmid#84005PurposepCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoterDepositorInsertswhite sgRNA-1
white sgRNA-1
UseCRISPRTagsExpressionMutationPromoterU6:1, U6:3, and dU6-1; dU6-3Available sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2
Plasmid#78606PurposepZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2DepositorInsertCMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2
UseAAV and CRISPRTagsHA tagExpressionMammalianMutationPromoter173CMV, U6Available sinceDec. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry
Plasmid#208834PurposeControl plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFPDepositorInsertmCherry
UseAAV and CRISPRTagsExpressionBacterial and MammalianMutationPromoterhSyn1Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-U6-gRNA2-CMV-eGFP
Plasmid#211760PurposePaired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, M. mulatta (rhesus macaque), Mouse)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-U6:gRNA
Plasmid#63156PurposeDestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6-PGK-hCD2
Plasmid#224294PurposeExpresses sgRNA from U6 promoter and human CD2 under PGKDepositorInsertU6 sgRNA cassette with CD2 under PGK promoter (CD2 Human)
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6/PGKAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNAs-Crym
Plasmid#200067PurposeFor the knock down of Crym with an astrocyte specific mCherry expressionDepositorInsertU6-3xsgRNA-Crym
UseAAV, CRISPR, and Mouse TargetingTagsGfaABC1D-mCherryExpressionMutationPromoterU6, GfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-GFP
Plasmid#200068PurposeFor the knock down of GFP with an astrocyte specific mCherry expressionDepositorInsertU6-sgRNA-GFP
UseAAV, CRISPR, and Mouse TargetingTagsGfaABC1D-mCherryExpressionMutationPromoterU6, GfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
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PlayBack-U6-Xba1
Plasmid#203300PurposeFor expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-empty
Plasmid#140580PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only