59,928 results

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mU6-sgRNA-CLTA-mCherry

Plasmid

#240637

Purpose

Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

puro-T2A-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDest eGFP MBNL1 42 kDa

Plasmid

#61277

Purpose

Mammalian expression of EGFP-tagged human MBNL1

Depositor

Nicolas Charlet-Berguerand

Insert

MBNL1 (MBNL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pIRES Centrin1 mCherry

Plasmid

#64338

Purpose

Bicistronic expression of mCherry-Centrin1 fusion and Hygro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1

Tags

IRES-Hygro and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLX304-ERBB2

Plasmid

#175846

Purpose

Expresses ERBB2

Depositor

Evan Braunstein

Article

Braunstein et al Leukemia. 2016 Nov;30(11):2242-2245. doi

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-ROR1

Plasmid

#116789

Purpose

Lentiviral expression of ROR1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ROR1 (ROR1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-182)-2xFv-Venus

Plasmid

#106077

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-182)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-182

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDest17_MAX

Plasmid

#234044

Purpose

Bacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage site

Depositor

Tanja Mittag

Article

Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

Insert

MAX (MAX Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG2-EGFP-C-IFT139

Plasmid

#218738

Purpose

Expresses C-terminally EGFP-tagged IFT139 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT139 (TTC21B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (IFT88, donor)

Plasmid

#80769

Purpose

Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

IFT88 gRNA#1 (IFT88 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_EML4:1-981

Plasmid

#211315

Purpose

Mammalian expression of full-length human EML4. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EML4:M1-S981 (EML4 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

I382V natural variant (VAR_031727)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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EBFP2-PCNA-19-SV40NLS-4

Plasmid

#55250

Purpose

Localization: Nucleus, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

3xSV40 NLS and EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-EGFP-C-IFT70B

Plasmid

#218729

Purpose

Expresses N-terminally EGFP-tagged IFT70B in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

Insert

IFT70B (TTC30B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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DMPK

Plasmid

#155550

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DMPK (DMPK Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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M51

Plasmid

#242620

Purpose

Strep-MYC-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-MYC-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_EML4:1-981

Plasmid

#211241

Purpose

Mammalian expression of full-length human EML4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EML4:M1-S981 (EML4 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

I382V natural variant (VAR_031727)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Mfn1-E209A

Plasmid

#141155

Purpose

Mammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysis

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

MFN 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

changed Glutamic acid at position 209 to Alanine …

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-hTERT-IRES-hygro

Plasmid

#85140

Purpose

Lentiviral expression of hTERT, used to create immortalized cell lines

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

hTERT (TERT Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only