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Addgene

We narrowed to 5,560 results for: chia

Showing: 41 - 80 of 5560 results
  1. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pDRM55 tet-AR(1-707)

    Plasmid
    #183503
    Purpose
    Lentiviral vector expressing a doxycycline-inducible constitutively active truncated androgen receptor (wild type AR)
    Depositor
    Insert
    Androgen receptor (AR Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Constitutively active truncated AR; spans AR 1-70…
    Promoter
    CMV
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pJK057

    Plasmid
    #75036
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes residues 2-7 to His (SSRNNP>HHHHHH)
    Promoter
    T7 and n/a
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pEG03

    Plasmid
    #154138
    Purpose
    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_gyrA
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pKSCTMF

    Plasmid
    #85073
    Purpose
    Gene insertion/deletion in Pichia pastoris
    Depositor
    Insert
    AOX1promoter - mazF - AOX1 tt- Zeocin R gene
    Use
    Gene insertion/deletion in pichia pastoris
    Tags
    Expression
    Mutation
    Promoter
    pAOX1
    Available since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pEG03'

    Plasmid
    #162578
    Purpose
    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_gyrA
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pEG01

    Plasmid
    #154136
    Purpose
    This plasmid carries fucO and aldA under the control of P_BAD promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_BAD
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pEG02

    Plasmid
    #154137
    Purpose
    This plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_thrC3
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pNC2

    Plasmid
    #72438
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg
    Promoter
    T7 and n/a
    Available since
    May 12, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. pCapV

    Plasmid
    #183028
    Purpose
    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Insert
    patatin-like phospholipase CapV
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid
    #111147
    Purpose
    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.
    Insert
    actin gamma 1 (ACTG1 Human)
    Use
    Pichia pastoris integration vector
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    codon-optimised for expression in Pichia pastoris
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pLY1244-T4gp44/62

    Plasmid
    #228205
    Purpose
    Expression in BL21(DE3) of WT T4 clamp loader consisting of gp44 and gp62
    Depositor
    Insert
    44/62
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pET26b-T4gp45

    Plasmid
    #228204
    Purpose
    Expression in BL21(DE3) of WT T4 clamp
    Depositor
    Insert
    45 (45 Escherichia phage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. pTYB3-T4gp43(WT)

    Plasmid
    #228203
    Purpose
    Expression in BL21(DE3) of WT T4 DNA polymerase
    Depositor
    Insert
    43
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. pET28b-T4Dda

    Plasmid
    #228215
    Purpose
    Expression in BL21(DE3) of T4 Dda-like helicase
    Depositor
    Insert
    dda (dda Escherichia phage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  17. pTYB1-T4denV

    Plasmid
    #228216
    Purpose
    Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme
    Depositor
    Insert
    denV
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Insert
    41 (41 Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  19. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Insert
    59 (59 Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. pTYB1-T4uvsX

    Plasmid
    #228213
    Purpose
    Expression in BL21(DE3) of T4 recombinase
    Depositor
    Insert
    uvsX (uvsX Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pTBY1-T4uvsY

    Plasmid
    #228214
    Purpose
    Expression in BL21(DE3) of T4 UvsY-like recombination mediator
    Depositor
    Insert
    uvsY (uvsY Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  23. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Insert
    43
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 80 of 5560 results