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Showing: 51 - 100 of 105 results

  1. pFRT-TODestFLAGHA_RBPMS_KDR_F27A

    Plasmid
    #65661
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    changed phenylalanine 27 to alanine (KDR backgrou…
    Promoter
    CMV, 2x Tet
    Available since
    June 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    65661-plasmid-map-sequence-id-111634

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  2. Tol2-kdrl:mCherry-caax

    Plasmid
    #194287
    Purpose
    Expression plasmid for zebrafish or Danionella cerebrum
    Depositor
    Pui-Ying Lam
    Article
    Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049
    Insert
    kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
    Use
    Zebrafish or danionella cerebrum expression
    Tags
    mCherryCAAX from the Tol2kit (Kwan et al., 2007)
    Expression
    Mutation
    Promoter
    kdrl(flk1)
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    194287-plasmid-map-sequence-id-382272

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  18. pD649-HAsp-KDR-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156967
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    KDR (KDR Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156967-plasmid-map-sequence-id-426821

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  19. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

    Plasmid
    #156414
    Purpose
    kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4B-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Expression
    Mutation
    mutant KRAS4B-G12V
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156414-plasmid-map-sequence-id-308707

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  20. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid
    #156415
    Purpose
    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4A-G12D (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mTagBFP2
    Expression
    Mutation
    mutant KRAS4A-G12D
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156415-plasmid-map-sequence-id-308711

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  21. JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

    Plasmid
    #156416
    Purpose
    kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4A-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mScarlet-I
    Expression
    Mutation
    mutant KRAS4A-G12V
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156416-plasmid-map-sequence-id-308708

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  34. TFORF1995

    Plasmid
    #144376
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KDR (KDR Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144376-plasmid-map-sequence-id-353178

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  35. mEmerald-VEGFR2-N

    Plasmid
    #54298
    Purpose
    Localization: Membrane Receptor, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    VEGFR2 (KDR Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54298-plasmid-map-sequence-id-372994

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  41. tRECs/3xUAS-FP attB

    Plasmid
    #220330
    Purpose
    encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)
    Depositor
    Erdem Bangi
    Inserts
    B3RT-STOP-B3RT-TagBFP-3xHA
    KDRT-STOP-KDRT-GFP-HSV
    FRTm71-STOP-FRTm71-mCherry
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    220330-plasmid-map-sequence-id-441802

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  43. CX3C1-DuET

    Plasmid
    #213217
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CX3C1 (CX3CR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213217-plasmid-map-sequence-id-430779

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  49. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    flk1
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  50. CX3C1-Tango

    Plasmid
    #66258
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CX3C1 (CX3CR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 51 - 100 of 105 results