Addgene

We narrowed to 1,457 results for: MBP

Showing: 51 - 100 of 1457 results

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
pHLmMBP-2

Plasmid

#72344

Purpose

Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Use

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Mutation

Promoter

CMV enhancer + chicken beta-actin promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBPCas2/3

Plasmid

#89238

Purpose

Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas2/3

Use

Tags

Strep II-MBP

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
40 pCOLI_K_MBP

Plasmid

#160183

Purpose

N-term MBP 3C cleavable Kan resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
11 pCOLI_A_MBP

Plasmid

#160247

Purpose

N-term MBP 3C cleavable Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
30 pCOLI_S_MBP

Plasmid

#160172

Purpose

N-term MBP 3C cleavable Spec resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DNMBP-6/6

Plasmid

#91489

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-6/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-6/6 (DNMBP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNMTMA_MBP_Z_Cav1_H6_Periplasmic_mScarlet

Plasmid

#223703

Purpose

To produce caveospheres with periplasmically encapsulated mScarlet

Depositor

Rob Parton

Article

T. D. et al bioRxiv

Insert

MBP_Z_Cav1_H6_Periplasmic_mScarlet

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SbMBP

Plasmid

#117861

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

SbMBP

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP2-2/3

Plasmid

#91468

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-2/3 (RIMBP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DNMBP-5/6

Plasmid

#91474

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-5/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-5/6 (DNMBP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only
pHH0103_DNMBP-2/6

Plasmid

#91422

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-2/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-2/6 (DNMBP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP2-3/3

Plasmid

#91411

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-3/3 (RIMBP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DNMBP-4/6

Plasmid

#91375

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-4/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-4/6 (DNMBP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP2-1/3

Plasmid

#91388

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-1/3 (RIMBP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP3-3/3

Plasmid

#91365

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3-3/3 (RIMBP3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP3-2/3

Plasmid

#91370

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3-2/3 (RIMBP3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP3-1/3

Plasmid

#91331

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3-1/3 (RIMBP3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP3B-1/1

Plasmid

#91299

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3B-1/1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DNMBP-3/6

Plasmid

#91277

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-3/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-3/6 (DNMBP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DNMBP-1/6

Plasmid

#91276

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-1/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-1/6 (DNMBP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 MBP_PrA/G-H6

Plasmid

#176077

Purpose

Express His6 tagged MBP_PrA/G in Escherichia coli

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

MBP PrA/G-H6

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Has Service

Cloning Grade DNA

Insert

LbCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-HisMBP

Plasmid

#11085

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SpyTagMBP

Plasmid

#35050

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyTagMBP

Use

Tags

His6

Expression

Bacterial

Mutation

Simple peptide tag added to the N-terminus of MBP…

Promoter

T7 promoter

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-hLIF

Plasmid

#179711

Purpose

Expresses H6-MBP-LIF

Depositor

Mark Arbing

Insert

Leukemia inhibitory factor (LIF Human)

Use

Tags

His6-MBP-TEV protease site

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-FnCas12a

Plasmid

#113432

Purpose

Expresses 10xHis-MBP fused to FnCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

FnCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-MS2

Plasmid

#65104

Purpose

can be used to isolate RNA binding complexes

Depositor

Josep Vilardell

Article

Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

Insert

MS2

Use

Tags

MBP

Expression

Bacterial

Mutation

double mutation (V75Q and A81G) that prevents oli…

Promoter

tac

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Use

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Mutation

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.RBBP4

Plasmid

#125164

Purpose

Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIDTSmart-MbPylRS

Plasmid

#217361

Purpose

Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

M. barkeri pyrrolysyl-tRNA synthetase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.EZH2

Plasmid

#125161

Purpose

Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EZH2 (EZH2 Human)

Use

Baculovirus expression vector

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-SpyCatcher

Plasmid

#79053

Purpose

Expresses maltose binding protein (MBP) SpyCatcher fusion

Depositor

James Bowie

Article

Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

Insert

MBP-SpyCatcher

Use

Tags

6xHis and Maltose binding protein

Expression

Bacterial

Mutation

Derived from pDEST14-SpyCatcher (Plasmid #35044)…

Promoter

T7

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

MBP promoter fragment (Mbp Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.dEZH2

Plasmid

#220241

Purpose

Expression of an EZH2 catalytic site mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

dEZH2 (EZH2 Human)

Use

Tags

6x His, MBP

Expression

Insect

Mutation

Y731F

Promoter

Polyhedrin

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4-p160MBP

Plasmid

#51

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 Myb binding protein (Mybbp1a Mouse)

Use

Tags

Gal4DBD

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2005

Availability

Academic Institutions and Nonprofits only
Flag-HA-STAMBP

Plasmid

#22560

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

STAMBP (STAMBP Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-AaCas12b

Plasmid

#113433

Purpose

Expresses Expresses 10xHis-MBP fused to AaCas12b

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AaCas12b

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.PHF19

Plasmid

#125168

Purpose

Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF19 (PHF19 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.PHF1

Plasmid

#125167

Purpose

Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF1 (PHF1 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-p160MBP

Plasmid

#41

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.AEBP2

Plasmid

#125165

Purpose

Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Zinc finger protein AEBP2 (AEBP2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EcMBP165-cpGFP.PPYF.T203V.pRSET

Plasmid

#33373

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

Improved MBP based Maltose Biosensor

Use

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EcMBP165-cpGFP.PPYF.pRSET

Plasmid

#33372

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

MBP based Maltose Biosensor

Use

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.JARID2

Plasmid

#125166

Purpose

Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

JARID2 (JARID2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-STAMBPL1

Plasmid

#22559

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

STAMBPL1 (STAMBPL1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.SUZ12

Plasmid

#125162

Purpose

Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein SUZ12 (SUZ12 Human)

Use

Baculovirus expression vector

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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