We narrowed to 126 results for: pj sac

1. pAV0772

   Plasmid

   #133516

   Purpose

   Expression of sfGFP tagged beta-tubulin to monitor microtubules

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(atb2)-sfGFP-atb2-terminator(atb2)-hphMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAV0607

   Plasmid

   #133504

   Purpose

   Expression of mCherry targeted to the plasma membrane with an amphipathic helix of RitC

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-mCherry-RitC-terminator(ScADH1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV0763

   Plasmid

   #133497

   Purpose

   Expression of nucleo-cytoplasmic mTagBFP2 in M-cells

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(mam1*)-mTagBFP2-terminator(ScADH1)-kanMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV0757

   Plasmid

   #133509

   Purpose

   Expression of 3GFP tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV0620

   Plasmid

   #133506

   Purpose

   Expression of eGFP tagged PCNA to monitor DNA replication

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV0785

   Plasmid

   #133507

   Purpose

   Expression of mCherry tagged PCNA to monitor DNA replication

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV0782

   Plasmid

   #133514

   Purpose

   Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV0751

   Plasmid

   #133485

   Purpose

   Expression in fission yeast from promoter pnmt1 , integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAV0752

   Plasmid

   #133486

   Purpose

   Expression in fission yeast from promoter pnmt41 , integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(nmt41)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAV0753

    Plasmid

    #133487

    Purpose

    Expression in fission yeast from promoter pnmt81 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV0783

    Plasmid

    #133515

    Purpose

    Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV0749

    Plasmid

    #133483

    Purpose

    Expression in fission yeast from promoter ptdh1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV0714

    Plasmid

    #133479

    Purpose

    Expression in fission yeast from promoter pact1, integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV0612

    Plasmid

    #133505

    Purpose

    Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV0750

    Plasmid

    #133484

    Purpose

    Expression in fission yeast from promoter purg1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(urg1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV0756

    Plasmid

    #133508

    Purpose

    Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV0764

    Plasmid

    #133520

    Purpose

    Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV0773

    Plasmid

    #133519

    Purpose

    Expression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV0835

    Plasmid

    #133512

    Purpose

    Expression of mCherry tagged CHD domain to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-mCherry-CHDRng2-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV0816

    Plasmid

    #133510

    Purpose

    Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV0747

    Plasmid

    #133481

    Purpose

    Expression in fission yeast from promoter ppom1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pom1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAV0748

    Plasmid

    #133482

    Purpose

    Expression in fission yeast from promoter ppak1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pak1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAV0605

    Plasmid

    #133503

    Purpose

    Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAV0746

    Plasmid

    #133480

    Purpose

    Expression in fission yeast from promoter prga3 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAct-GST-H3

    Plasmid

    #182842

    Purpose

    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    GST-histone H3 fusion
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAV0584

    Plasmid

    #133471

    Purpose

    Integration into fission yeast genome at ura4 locus with natMX selection cassette

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAV0585

    Plasmid

    #133472

    Purpose

    Integration into fission yeast genome at ade6 locus with hphMX selection cassette

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAV0586

    Plasmid

    #133473

    Purpose

    Integration into fission yeast genome at lys3 locus with bsdMX selection cassette

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAV0784

    Plasmid

    #133475

    Purpose

    Integration into fission yeast genome at his5 locus with bsdMX selection cassette

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAV0587

    Plasmid

    #133474

    Purpose

    Integration into fission yeast genome at his5 locus with bleMX selection cassette

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLIB-HIS-TEV-scCAK1

    Plasmid

    #177014

    Purpose

    Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDEST/LIfeAct-mCherry-N1

    Plasmid

    #40908

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    LifeAct
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    No stop codon to create c-terminal fusion

    Promoter

    CMV

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSW149

    Plasmid

    #122248

    Purpose

    pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

    Depositor

    Sarah Walker

    Article

    Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

    Insert

    TIF4631 (TIF4631 Budding Yeast)
    

    Use

    Tags

    Intein-Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLIB-HIS-TEV-Cyclin-B

    Plasmid

    #177012

    Purpose

    Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PSD-95 FLAG

    Plasmid

    #15463

    Depositor

    Wei-dong Yao

    Article

    Zhang et al J Biol Chem. 2007 May 25. 282(21):15778-

    Insert

    PSD-95 (Dlg4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Flag-Fos S374A

    Plasmid

    #118302

    Purpose

    Expression of mouse C-Fos

    Depositor

    Philip Stork

    Article

    Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

    Insert

    C-Fos S374A (Fos Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S374A

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Flag-Fos S362DS374A

    Plasmid

    #118305

    Purpose

    Expression of mous C-Fos

    Depositor

    Philip Stork

    Article

    Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

    Insert

    C-Fos S362DS374A (Fos Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S362DS374A

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Flag-Fos S362AS374D

    Plasmid

    #118304

    Purpose

    Expression of mouse C-Fos

    Depositor

    Philip Stork

    Article

    Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

    Insert

    C-Fos S362AS374D (Fos Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S362AS374D

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Flag-Fos S362D

    Plasmid

    #118303

    Purpose

    Expression of mouse C-Fos

    Depositor

    Philip Stork

    Article

    Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

    Insert

    C-Fos S362D (Fos Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S362D

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLIB-GST-3C-CDK1

    Plasmid

    #177011

    Purpose

    Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Tags

    GST-3C

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBDP

    Plasmid

    #217827

    Purpose

    Stuffer plasmid with golden gate compatible bidirectional promoter

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    Gal1/Gal10 Bidirectional Promoter
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-SaPE2

    Plasmid

    #174817

    Purpose

    Mammalian expression of SaCas9 prime editor 2

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLIB-HIS-TEV-CKS1

    Plasmid

    #177013

    Purpose

    Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CKS1 (CKS1B Synthetic, Human)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. hSyn-GW-dCas9-SET2-IRES2-mCherry

    Plasmid

    #202089

    Purpose

    Neuron-specific expression vector of dCas9-Set2

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2
    

    Use

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMGF172

    Plasmid

    #96998

    Purpose

    ish1 mCherry insertion cassette with ura4+ gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + ura4(…

    Promoter

    N/A

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMGF130

    Plasmid

    #96971

    Purpose

    S. pombe lem2 knockout cassette with hygro resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    lem2 (lem2 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    whole ORF deletion and replaced with hygro resist…

    Promoter

    N/A

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMGF157

    Plasmid

    #96972

    Purpose

    ish1-mCherry insertion cassette with Hygro resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + hygro…

    Promoter

    N/A

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pMGF170

    Plasmid

    #96997

    Purpose

    ish1-mCherry insertion cassette with NAT resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + NAT r…

    Promoter

    N/A

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

    Plasmid

    #202090

    Purpose

    Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2(R195G, C201A)
    

    Use

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Mutation

    R195G and C201A abolish catalytic activity

    Promoter

    human Synapsin

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMGF169

    Plasmid

    #96996

    Purpose

    ish1 mCherry insertion cassette with G418 resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + G418 …

    Promoter

    N/A

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge