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We narrowed to 345 results for: pET3

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pET30a-AlkB

Plasmid

#79050

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Use

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET30-RT1306

Plasmid

#131521

Purpose

Expresses engineered RT-1306 with His-tag

Depositor

Bryan Dickinson

Article

Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

Insert

RT-1306

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET32a-HD46Q

Plasmid

#11515

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 46Q glutamines (HTT Human)

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 46 glutamines, vector m…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pET32-MCM2/7

Plasmid

#116949

Purpose

Expresses human MCM2 and human MCM7

Depositor

James Chong

Article

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM7 (MCM7 Human)
Human MCM2 (MCM2 Human)

Use

Tags

Thioredoxin, His6

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-HD25Q

Plasmid

#11508

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 25 glutamines (HTT Human)

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 25 glutamines, vector m…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-HD16Q

Plasmid

#11487

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 16 glutamines (HTT Human)

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 16 glutamines, vector m…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET31b-T7-Spinach2

Plasmid

#79783

Purpose

Expresses Spinach2 under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET30-2-PHGDH

Plasmid

#83907

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Tags

6x His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pET30a(+)-PE2

Plasmid

#180015

Purpose

Expressing PE2 protein in E.coli for protein purification

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Lac operator-controlled T7 promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET3a-Yuh1

Plasmid

#18895

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pET30-2-PSAT1

Plasmid

#83908

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine aminotransferase 1 (PSAT1 Human)

Use

Tags

6x His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-K63R

Plasmid

#18898

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin-K63R (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Lysine 63 is replaced with a Argnine residue

Promoter

Available since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pET30a-Tn5

Plasmid

#127917

Purpose

Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Tn5

Use

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pET303-colE1T

Plasmid

#155180

Purpose

Expresses colicin E1 residues 1-190

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-190

Use

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-190

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-colE1TR

Plasmid

#155181

Purpose

Expresses colicin E1 residues 1-364

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-364

Use

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-364

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET32-sLatherin

Plasmid

#61685

Purpose

bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

Depositor

Brian Smith

Article

Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

Insert

Latherin (LATH horse)

Use

Tags

HIS and Trx-Tag thioredoxin protein

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

T7

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pET30b-7Y-SrtA

Plasmid

#105601

Purpose

expresses 7Y sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7Y mutant

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET30-2-GPD1

Plasmid

#83911

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)

Use

Tags

6x His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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