Skip to main content
Addgene
Showing: 381 - 400 of 772 results

  1. pMSP1D1G1

    Plasmid
    #97325
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1G1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of G residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97325-plasmid-map-sequence-id-182623

    Enlarge

  3. pFastBac HT JS-Munc18b

    Plasmid
    #135554
    Purpose
    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18b (Stxbp2 Rat)
    Use
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    March 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    135554-plasmid-map-sequence-id-272494

    Enlarge

  4. pMSP1D1T1

    Plasmid
    #97327
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of T residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97327-plasmid-map-sequence-id-182625

    Enlarge

  5. pMSP1D1T2

    Plasmid
    #97328
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T2
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of two T residues after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97328-plasmid-map-sequence-id-182618

    Enlarge

  7. 5E26 (PANK2)

    Plasmid
    #79516
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK2 (PANK2 Human)
    Use
    Baculovirus expression
    Tags
    6xHis and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79516-plasmid-map-sequence-id-150092

    Enlarge

  8. PREX1

    Plasmid
    #32041
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PREX1 (PREX1 )
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    first PDZ domain only (amino acids Residues 607-7…
    Promoter
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. MLH1

    Plasmid
    #32859
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1 (MLH1 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    contains amino acids 486-751
    Promoter
    Available since
    Feb. 22, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. human KANK2 Ankyrin Repeats pET151

    Plasmid
    #164054
    Purpose
    Expresses human KANK2 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164054-plasmid-map-sequence-id-327671

    Enlarge

  12. pET22b-EC12WTC9AAvi

    Plasmid
    #47925
    Purpose
    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.
    Depositor
    Tanja Kortemme
    Article
    Ritterson et al J Am Chem Soc. 2013 Aug 7.
    Insert
    E-Cadherin EC12
    Use
    Tags
    6xHis, Avi Tag, and TEV Cleavage
    Expression
    Bacterial
    Mutation
    changed Cysteine 9 to Alanine
    Promoter
    T7
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47925-plasmid-map-sequence-id-68712

    Enlarge

  14. CBX7

    Plasmid
    #25241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX7_04 (CBX7 Human)
    Use
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. JMJD1CA-c089

    Plasmid
    #79644
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD1C (JMJD1C Human)
    Use
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79644-plasmid-map-sequence-id-150204

    Enlarge

  17. CBX4

    Plasmid
    #25237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX4_02 (CBX4 Human)
    Use
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. GALTA-c001

    Plasmid
    #79645
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GALT (GALT Human)
    Use
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79645-plasmid-map-sequence-id-150209

    Enlarge

Showing: 381 - 400 of 772 results