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Showing: 761 - 800 of 84239 results

  4. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

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  11. pEGFP-Rab21-CCSS

    Plasmid
    #83424
    Purpose
    Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.
    Depositor
    Johanna Ivaska
    Article
    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.
    Insert
    Rab21 (Rab21 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Residues 218 and 219 from C to S (CCSS: TGCTGT -&…
    Promoter
    CMV
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83424-plasmid-map-sequence-id-166315

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  21. pSBL_DK202

    Plasmid
    #156426
    Purpose
    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Use
    Tags
    EGFP-His
    Expression
    Mammalian
    Mutation
    aa 42-275
    Promoter
    CMV
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156426-plasmid-map-sequence-id-306303

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  27. TagBFP(S2h)3(S4h)3-gs-P6f

    Plasmid
    #228083
    Purpose
    Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers, N-term TagBFP, and C-term P6f; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h, S4h, P6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228083-plasmid-map-sequence-id-454691

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  28. pKM 1996

    Plasmid
    #217825
    Purpose
    Expresses putative photoreceptor SA-phr1 from S. alba. This gene shows high homology to the phr genes in prokaryotes.
    Depositor
    Aziz Sancar
    Article
    Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9.
    Insert
    SA-phr1
    Use
    Tags
    Maltose-binding protein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217825-plasmid-map-sequence-id-434879

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Showing: 761 - 800 of 84239 results