We narrowed to 32,226 results for: Eng
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Plasmid#126710Purposeyeast plasmid complementing ADH3 from Komagataella phaffiiDepositorInsertadh3
Tags6xHis,MycExpressionYeastPromoterGAPAvailable SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_lacO-cP-AOX1-sAR
Plasmid#126743Purposeyeast plasmid overexpressing CPR and sLovADepositorInsertslovA,cpr
Tags6xHisExpressionYeastAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPrps3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPeft2AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPsmtef1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPsptdh3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppgk1-PYC-Ttpi1
Plasmid#127735PurposeYeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyExpressionYeastPromoterPpgk1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppxr1-PYC-Tnat1
Plasmid#127736PurposeYeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyExpressionYeastPromoterPpxr1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Psbtdh3-CSC-Tefm1
Plasmid#127742PurposeYeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyExpressionYeastPromoterPsbtdh3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Peno2-ACDH-Ttal1
Plasmid#127743PurposeYeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyExpressionYeastPromoterPeno2AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Pvma6-ACDH-Trps14a
Plasmid#127744PurposeYeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyExpressionYeastPromoterPvma6AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Prpl28-ACDH-Tecm10
Plasmid#127745PurposeYeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyExpressionYeastPromoterPrpl28AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Ptdh3-ACDH-Trpl3
Plasmid#127746PurposeYeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyExpressionYeastPromoterPtdh3AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1
Plasmid#222339PurposePlasmid for expressing evolved and engineered Bxb1 in the mammalian cellsDepositorInsertCodon-optimized evolved and engineered Bxb1
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA1 with trimmed attP
Plasmid#222346PurposepegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA1
UseCRISPRAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pREDCas9
Plasmid#71541PurposeConstitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
lambda red genes
plasmid curing system
ExpressionBacterialPromoterpBADAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1
Plasmid#222337PurposePlasmid for expressing wild type Bxb1 in mammalian cellsDepositorInsertCodon-optimized wild type Bxb1
ExpressionMammalianPromoterCMVAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialPromoterPlacUV5Available SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTUM4
Plasmid#230905PurposePlasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coliDepositorInsertsExpressionBacterialAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
amajLime chromoprotein
Plasmid#117843PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coliDepositorInsertpromoter, RBS, amajLime
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
fwYellow chromoprotein
Plasmid#117841PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coliDepositorInsertpromoter, RBS, fwYellow
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
scOrange chromoprotein
Plasmid#117840PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coliDepositorInsertpromoter, RBS, scOrange
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilGFP chromoprotein
Plasmid#117842PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilGFP
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
aeBlue chromoprotein
Plasmid#117846PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, aeBlue
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS3Clux
Plasmid#55172Purposelux-reporter vector, integration at sacA, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxPromoternoneAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS2E
Plasmid#55169PurposeEmpty vector, integration at lacA, ampr, mlsrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxPromoternoneAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS1ClacZ
Plasmid#55171PurposelacZ-reporter vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxPromoternoneAvailable SinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-Nme2ABE8e
Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertNme2ABE8e
TagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-SauriABE8e
Plasmid#189927PurposePlasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertSauriABE8e
ExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-CjABE8e
Plasmid#189929PurposePlasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertCjABE8e
ExpressionMammalianMutationCjCas9 D8APromoterCMVAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
tsPurple chromoprotein
Plasmid#117848PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coliDepositorInsertpromoter, RBS, tsPurple
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilCP_Pink chromoprotein
Plasmid#117851PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP_Pink
UseSynthetic Biology; Escherichia coliMutation64A,65C, BioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
gfasPurple chromoprotein
Plasmid#117849PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coliDepositorInsertpromoter, RBS, gfasPurple
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
spisPink chromoprotein
Plasmid#117839PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coliDepositorInsertpromoter, RBS, spisPink
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilCP_Orange chromoprotein
Plasmid#117850PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP_Orange
UseSynthetic Biology; Escherichia coliMutation64V,65G, BioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
evoBxb1_IVT
Plasmid#222341PurposePlasmid for evolved Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved Bxb1
UseTemplate for in vitro transcriptionAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein
Plasmid#117838PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coliDepositorInsertpromoter, RBS, asPink
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialPromoterPlacUV5Available SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
eforRed chromoprotein
Plasmid#117837PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coliDepositorInsertpromoter, RBS, eforRed
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1_IVT
Plasmid#222340PurposePlasmid for wild type Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized wtBxb1
UseTemplate for in vitro transcriptionPromoterT7 (inactivated)Available SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS4S
Plasmid#55170PurposeEmpty vector, integration at thrC, ampr, specrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxPromoternoneAvailable SinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
meffBlue chromoprotein
Plasmid#117845PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, meffBlue
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialPromoterT5-lacAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NG-PE2-BSD
Plasmid#176933PurposeExpresses PE2-NG variant in mammalian cellsDepositorInsertPE2-NG variant
UseLentiviralTagsSV40 NLSExpressionMammalianPromoterEFSAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
cjBlue chromoprotein
Plasmid#117844PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, cjBlue
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
meffRed chromoprotein
Plasmid#117836PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coliDepositorInsertpromoter, RBS, meffRed
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-BE3.9max C-terminal
Plasmid#137172PurposeExpresses the C-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorInsertNpu-BE3.9max_C-terminal
ExpressionMammalianPromoterCMVAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax C-terminal
Plasmid#137174PurposeExpresses the C-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_C-terminal
ExpressionMammalianPromoterCMVAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
amilGFP chromoprotein promoter-less
Plasmid#118006PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilGFP
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only