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We narrowed to 1,094 results for: gibson assembly

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Showing: 951 - 1000 of 1094 results

  1. pBD21_034

    Plasmid
    #109151
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    TagBFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    109151-plasmid-map-sequence-id-220104

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  2. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

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  3. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

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  5. pHRSV_CHARMS-2xTIR

    Plasmid
    #233930
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-2xTIR which contain a tandem dimer of TIR domains
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-2xTIR
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233930-plasmid-map-sequence-id-484962

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  7. pHRSV_CHARMS-bDD-5xT6BMs

    Plasmid
    #233939
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-bDD with 5 TRAF6 binding motifs
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-bDD-5xT6BMs
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233939-plasmid-map-sequence-id-469210

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  8. pHRSV_CHARMS-V43D/E52K/R62E

    Plasmid
    #233940
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS three mutations in the death domain that disrupt oligomer formation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-V43D/E52K/R62E
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233940-plasmid-map-sequence-id-469200

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  9. pHRSV_CHARMS-1x

    Plasmid
    #233923
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-1x
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233923-plasmid-map-sequence-id-469219

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  10. pHRSV_CHARMS-1xA

    Plasmid
    #233924
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-1xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233924-plasmid-map-sequence-id-468361

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  11. pHRSV_CHARMS-3x

    Plasmid
    #233925
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-3x
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233925-plasmid-map-sequence-id-469216

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  12. pHRSV_CHARMS-3xA

    Plasmid
    #233926
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-3xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233926-plasmid-map-sequence-id-469198

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  13. pHRSV_CHARMS-5x

    Plasmid
    #233927
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-5x
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233927-plasmid-map-sequence-id-469202

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  14. pHRSV_CHARMS-5xA

    Plasmid
    #233928
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which contains only 5 TRAF6 binding motifs with a alanine mutation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-5xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233928-plasmid-map-sequence-id-468363

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  15. pHRSV_CHARMS-S34Y

    Plasmid
    #233929
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-S34Y which contains a mutation in the death domain that disrupt oligomer formation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-S34Y
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233929-plasmid-map-sequence-id-469194

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  16. pHRSV_CHARMS-11xTA

    Plasmid
    #233932
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domain
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-11xTA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233932-plasmid-map-sequence-id-469197

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  17. pBD21_035

    Plasmid
    #109152
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    EGFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109152-plasmid-map-sequence-id-209236

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  18. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid
    #196974
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Article
    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Tags
    GST and mNeonGreen
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196974-plasmid-map-sequence-id-388923

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  25. pNC-Y

    Plasmid
    #238998
    Purpose
    Node C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Article
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sgRNA-1 downstream of a weak constitutive promoter and a binding site for sgRNA-2
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    238998-plasmid-map-sequence-id-479360

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  29. MZB164

    Plasmid
    #217842
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    HaloTag
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217842-plasmid-map-sequence-id-433000

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  32. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  40. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  41. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  42. p2R3z-BASI-ccdB-BSAI

    Plasmid
    #118389
    Purpose
    Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4
    Insert
    ccdB
    Use
    Unspecified
    Mutation
    See Depositor Comments Section
    Available Since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    118389-plasmid-map-sequence-id-470418

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  44. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  45. pNB-Rep

    Plasmid
    #238996
    Purpose
    Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Article
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    238996-plasmid-map-sequence-id-495027

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Showing: 951 - 1000 of 1094 results