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Plasmid#169718PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. TYR and PHE shuffled into 5 clusters.DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD Aro-
Plasmid#169715PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD Aro+
Plasmid#169717PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD Patchy
Plasmid#169719PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Δ1-60MEK1C222
Plasmid#164641PurposeBacterial expression plasmid for His6-ΔMEK1C222DepositorInsertN-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationS222C/C277S/C376S; residues 1-60 at N-terminus we…PromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Δ1-60MEK1C218
Plasmid#164643PurposeBacterial expression plasmid for His6-ΔMEK1C218DepositorInsertN-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationS218C/C277S/C376S; residues 1-60 at N-terminus we…PromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Δ1-60MEK1C218C222
Plasmid#164645PurposeBacterial expression plasmid for His6-ΔMEK1C218C222DepositorInsertN-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationS218C/S222C/C277S/C376S; residues 1-60 at N-termi…PromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS1132
Plasmid#29053PurposeHigh copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP/Cre fusion reporterDepositorInsertPle67 (FEV Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-GenRep
Plasmid#214674PurposeMAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exonsDepositorInsertMAPT (MAPT Human)
UseTagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9 and Exon 11, with a BamHI EcoRI c…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19_18GZG
Plasmid#117228Purposeshble flanked by an endogenous GAPDH promoter and terminator systemDepositorInsertshble
UseThraustochytrid expressionTagsExpressionMutationPromoterGAPDHAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-neo
Plasmid#155026PurposeDinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) geneDepositorInsertamino 3'-glycosyl phosphotransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-pat
Plasmid#155028PurposeDinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, geneDepositorInsertPhosphinothricin N-acetyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-bsr
Plasmid#155027PurposeDinoflagelate backbone for blasticidin S-resistance gene (bsr)DepositorInsertBlasticidin-S deaminase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC:FCP:ShBle:FCP:EGFP
Plasmid#85987PurposeExpresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.DepositorInsertsShBle
EGFP
UseTagsExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUB2.1
Plasmid#40728PurposeModified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert backgroundDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOpen-TthRT
Plasmid#165566PurposeDNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2DepositorInsertThermus thermophilus (Tth) RT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAST-3x attB
Plasmid#220329Purposeincludes 3 standard UAS cassettesDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mTagBFP2 PuroR
Plasmid#101893PurposeLentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 PuroR
Plasmid#84386PurposeLentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[Tc'hsp5'3'UTR]fa
Plasmid#86450PurposeHeat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)DepositorInsertTc'hsp5'3'UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only