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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,175 results for: nls

Showing: 381 - 400 of 6175 results

  5. GPKAnls

    Plasmid
    #61094
    Purpose
    GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal
    Depositor
    Steven Green
    Article
    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.
    Insert
    pkac2
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pCAGEN_V5_eMagAF_C74_cMycNLS

    Plasmid
    #235640
    Purpose
    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi C-terminal fragment
    Use
    Tags
    V5-eMagAF and cMycNLS
    Expression
    Mammalian
    Mutation
    residues 74-321
    Promoter
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235640-plasmid-map-sequence-id-467541

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  15. pGGD_RT_li_Tq2CFP_2NLS

    Plasmid
    #136972
    Purpose
    Goldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136972-plasmid-map-sequence-id-269108

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  16. pGGD_RT_2NLS

    Plasmid
    #136977
    Purpose
    Goldengate/Greengate module nuclear localization signal
    Depositor
    Klaus Theres
    Insert
    SV40 NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136977-plasmid-map-sequence-id-269089

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  17. MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid
    #176628
    Purpose
    TF A self-activation cassette in MultiFate-2.3
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176628-plasmid-map-sequence-id-347271

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  18. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176624
    Purpose
    TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176624-plasmid-map-sequence-id-347134

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  19. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid
    #114084
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.4
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    Use
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimiā€¦
    Promoter
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114084-plasmid-map-sequence-id-230536

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  20. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

    Plasmid
    #197029
    Purpose
    AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized ecDHFR-enOsCas12f1-ecDHFR
    Use
    AAV
    Tags
    Expression
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197029-plasmid-map-sequence-id-388970

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Showing: 381 - 400 of 6175 results