164,577 results

1. mRFP-UtrCH

   Plasmid

   #26739

   Depositor

   William Bement

   Article

   Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

   Insert

   Utrophin
   

   Use

   Reporter for f-actin

   Tags

   monomeric RFP

   Expression

   Mammalian

   Mutation

   Calponin homology domain of utrophin (aa# 1-261)

   Promoter

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-mTagBFP-CAAX

   Plasmid

   #75149

   Purpose

   Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mTagBFP-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKL002

   Plasmid

   #98921

   Purpose

   pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

   Depositor

   Joel Kralj

   Article

   Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

   Inserts

   PROPS
   GCaMP6f
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH3U3-zif268 (omega)

   Plasmid

   #18046

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   zif268 binding site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The preferred binding site for zif268 is inserted…

   Promoter

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUW-VenusFlag-hCD44

   Plasmid

   #211824

   Purpose

   Express CD44 in mammalian cells

   Depositor

   Haian Fu

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   CD44 (CD44 Human)
   

   Use

   Lentiviral

   Tags

   N-Terminal Venus and Flag tags

   Expression

   Mammalian

   Mutation

   isoform corresponds to UniProt ID P16070-1 with a…

   Promoter

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZT-AAVS1-R1

   Plasmid

   #52638

   Purpose

   TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

   Depositor

   Jizhong Zou, Mahendra Rao

   Article

   Luo et al Stem Cells Transl Med. 2014 May 15. pii:

   Insert

   Right AAVS1 TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-PE-bacteria

   Plasmid

   #172715

   Purpose

   Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   Cas9n-linker-M-MLV2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H840A of Cas9

   Promoter

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMP71-super-IL-2

   Plasmid

   #174599

   Purpose

   Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZT-AAVS1-L1

   Plasmid

   #52637

   Purpose

   TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

   Depositor

   Jizhong Zou, Mahendra Rao

   Article

   Luo et al Stem Cells Transl Med. 2014 May 15. pii:

   Insert

   Left AAVS1 TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-OMM-jRCaMP1b

    Plasmid

    #127871

    Purpose

    Red-shifted fluorescent reporter for mitochondrial outer membrane Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_LMX1A_P2A_Hygro_Barcode

    Plasmid

    #120457

    Purpose

    Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    LMX1A (LMX1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-CMV-Flag-GFP_IDG-K

    Plasmid

    #135275

    Purpose

    Gateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-GFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ10873

    Plasmid

    #183961

    Purpose

    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIX_403

    Plasmid

    #41395

    Purpose

    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3990 pET ubcH8

    Plasmid

    #8650

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

    Insert

    UbcH8 (UBE2L6 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pCold TDP43 WT

    Plasmid

    #27451

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa, GST, and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET20b_Pa-PiuA

    Plasmid

    #128955

    Purpose

    expresses Pseudomonas aeruginosa PiuA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28c-F30-Broccoli

    Plasmid

    #66788

    Purpose

    Expresses Broccoli in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Broccoli in the F30 scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPRi(v2)-Blast

    Plasmid

    #170068

    Purpose

    Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFGAMS-EGFP

    Plasmid

    #99107

    Purpose

    Expresses human PFAS-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PFAS (PFAS Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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