165,075 results

1. pC13N-dCas9-BFP-KRAB

   Plasmid

   #127968

   Purpose

   constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-dCas9-NLS-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-HSPA8

   Plasmid

   #121161

   Purpose

   Mammalian expression of GFP tagged HSPA8

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   HSPA8 (HSPA8 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GPR87-DuET

   Plasmid

   #213293

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR87 (GPR87 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ornithine transcarbamylase (OTC)

   Plasmid

   #71877

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC (Otc Rat)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nuclear Myosin I YFP

   Plasmid

   #60241

   Purpose

   Expresses nuclear isoform of myosin IC attached to YFP and an NLS

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. IGF2BP1

   Plasmid

   #155676

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   IGF2BP1 (IGF2BP1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE GFP

   Plasmid

   #10668

   Purpose

   Empty backbone for retroviral gene expression. Select recipient cells using GFP.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6P-1_WT RAD23B

   Plasmid

   #201457

   Purpose

   Expresses human RAD23B with an N-terminal GST tag in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   tac promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSpCas9(1.1)_No_FLAG_AAVS1_T2

    Plasmid

    #79888

    Purpose

    Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3-EF1aH c-Met

    Plasmid

    #86498

    Purpose

    Mammalian expression of human c-Met

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRL_SRSF2_WT_mCherry

    Plasmid

    #84020

    Purpose

    expression of SRSF2 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    2TA-mCherry and Flag

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRXIP GFP-Stx7

    Plasmid

    #45921

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    syntaxin 7 (STX7 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pEGFR-PATagRFP

    Plasmid

    #31950

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Tags

    EGF receptor (EGFR)

    Expression

    Mammalian

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    CMV

    Available Since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-PM-RA-BlastR

    Plasmid

    #211708

    Purpose

    Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    PM-targetted RA (Lck Synthetic)
    

    Use

    Lentiviral

    Tags

    RA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p35S_GFP-GUS

    Plasmid

    #167122

    Purpose

    Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    GFP-GUS
    

    Tags

    mGFP5, GUS

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. L1_lacZgRNA-Ck2

    Plasmid

    #136137

    Purpose

    L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS Omomyc

    Plasmid

    #113164

    Purpose

    Omomyc expression in mammalian cells

    Depositor

    Sergio Nasi

    Article

    Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

    Insert

    Omomyc (MYC Human)
    

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pscALPSpuro-MmACE2 (Mouse)

    Plasmid

    #158087

    Purpose

    Expresses mouse ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Mouse ACE2 (Ace2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUG6SP-tTA'

    Plasmid

    #41030

    Depositor

    Michael Nick Boddy

    Article

    Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

    Insert

    tTA' (tetR'-VP16)
    

    Available Since

    Dec. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. spNW100

    Plasmid

    #173488

    Purpose

    Expresses spNW100 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW100
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    P1049L- please see depositor comments

    Promoter

    T7

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pInducer20-Blast

    Plasmid

    #109334

    Purpose

    pInducer20 with added blasticidin resistance

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    Lentiviral; Inducible expression

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. IronFistMUT.

    Plasmid

    #243008

    Purpose

    Iron binding deficient IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    hemerythrin-like-mNeonGreen (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    H15A, H57A, E58A, E61A, H126A, E130A

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. Iron Insensitive Control Construct 2

    Plasmid

    #243007

    Purpose

    Iron Insensitive Control Construct under the same expression promoter used in IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    mNeonGreen
    T2A
    mCherry
    

    Use

    Gateway

    Expression

    Mammalian

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. IronFist

    Plasmid

    #243006

    Purpose

    Genetically encoded fluorescent iron reporter

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    Hemerythrin-like (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3-ATR WT

    Plasmid

    #31611

    Depositor

    Aziz Sancar

    Article

    Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

    Insert

    ATR (ATR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. TetO-FUW-Lhx2

    Plasmid

    #61537

    Purpose

    Expresses Lhx2 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Lhx2 (Lhx2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRK5-HA-Ubiquitin-K6

    Plasmid

    #121151

    Purpose

    Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    All Ks apart from K6 mutated to Rs

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMD2-VSVGmut

    Plasmid

    #182229

    Purpose

    Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    VSVGmut
    

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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