161,123 results
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Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.155_LV-Puro-T2A-mTagBFP2
Plasmid#205553PurposeExpress puromycin resistance marker and mTagBFP2DepositorInsertPuro-T2A-mTagBFP2
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-TBP
Plasmid#26674DepositorInsertTBP (TBP Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
dPspCas13b-mutFTO
Plasmid#223697PurposeExpressing fusion protein of dPspCas13b and inactive mutant of human FTODepositorInsertdPspCas13b-GGS-mutFTO (FTO Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLOVE-Sox2
Plasmid#15953DepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationThe recombination reaction required to clone &quo…PromoterAvailable sinceNov. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Calreticulin
Plasmid#191940PurposeExpression of calreticulinDepositorInsertCalreticulin (Calr Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
TPC1-mCherry
Plasmid#135182PurposeExpresses TPC1 with C-terminus mCherry tag in mammalian cellsDepositorInsertTPC1 (Tpcn1 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLOVE empty vector
Plasmid#15948DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
Puro-Cas9 donor
Plasmid#58409PurposeDonor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locusDepositorInserthSpCas9
UseCRISPRTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-mRipk3
Plasmid#78805PurposeTo overexpress mRipk3 in Mammalian CellsDepositorInsertRIPK3 (Ripk3 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF1-3'UTR
Plasmid#136036PurposeUPF1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (TTATTACCCAGAATAAGATGC)DepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lapronic/pcDNA3.1(-)
Plasmid#140756PurposeFRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate RatioDepositorInsertLapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST_119_LVL2 cam
Plasmid#179333PurposeNT-CRISPR plasmid for integration of multiple gRNAs.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290
Plasmid#197989PurposeFor the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.DepositorInsertHSALNT0217290
UseLentiviralTagslacExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide
Plasmid#117986PurposepLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.DepositorInsertsgRNA backbone (F+E)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV_hEF1a_rtTA3
Plasmid#61472Purpose3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)DepositorInsertrtTA3
UseLentiviralTagsExpressionMutationPromoterhEF1aAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-GST-4EBP1-Low GC
Plasmid#69018Purposeexpresses tagged 4EBP1DepositorInsertEIF4EBP1 (EIF4EBP1 Human)
UseTagsGST and HAExpressionMammalianMutationlowGC content, wt is ~70% GC rich. Still, it code…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRKH3-slpGFP
Plasmid#27168DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11
Plasmid#22901DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK11 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-mCherry
Plasmid#217005Purposelentiviral plasmid for gRNA cloning with selectable mCherry expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX459
Plasmid#229214PurposeCRISPR array plasmid for MAP1B (Spacers 1-24)DepositorInsertCRISPR array plasmid for MAP1B (Spacers 1-24)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Kif2c
Plasmid#208651PurposeMammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoterDepositorInsertKif2c (KIF2C Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Trx-mSA2
Plasmid#52320PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, S tag, TEV site, and ThioredoxinExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.GFP
Plasmid#57818PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEFS and hU6Available sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
UseTags14xHis-Avi-SUMOEuExpressionBacterialMutationPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyric-mCherry
Plasmid#226563PurposeEncodes Lyric protein labeled with mCherryDepositorInsertLyric (Lysine-rich CEACAM1 coisolated) (MTDH Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pN1/G3BP1-iRFP
Plasmid#129339PurposeStress granule markerDepositorInsertiRFP (from Dr. M. Matsuda)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only