We narrowed to 10,805 results for: CHL

1. pEPQD1CB0122

   Plasmid

   #177054

   Purpose

   MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEPQD1CB0118

   Plasmid

   #177050

   Purpose

   MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   iridoid synthase (CrISY) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPQD1CB0116

   Plasmid

   #177048

   Purpose

   MoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPQD1CB0114

   Plasmid

   #177046

   Purpose

   MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geraniol 8-oxidase (CrG8H) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PROM5_MpU6

   Plasmid

   #136120

   Purpose

   Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM5_MpU6
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CDS_Cas9-NLS

   Plasmid

   #136121

   Purpose

   Puchta Arabidopsis codon-optimised Cas9 for CRISPR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS_AtcoCas9-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CDS12-MpER-Targ

   Plasmid

   #136094

   Purpose

   CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   ER targeting peptide from predicted chitinase Mapoly0069s0092
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. CTAG_eGFP-GHtag-HDEL

   Plasmid

   #136096

   Purpose

   FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEPQD1CB0112

   Plasmid

   #177044

   Purpose

   MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geraniol synthase (CrGES) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAP12008

    Plasmid

    #177036

    Purpose

    Level 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCAT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    Cauliflower Mosaic Virus (CaMV) 35S promoter and Tobacco Mosaic Virus (TMV) omega 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPQD0CM0061

    Plasmid

    #177026

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    iridoid oxidase; CYP76A26
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    amplified from cDNA, contains T359S relative to K…

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0CM0818

    Plasmid

    #177020

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase; geranyl diphosphate synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    transit peptide removed

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PdnaK-IR3 GFP pZa

    Plasmid

    #170084

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PdnaK-IR3

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165888

    Purpose

    Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165889

    Purpose

    Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. P[acman]-Omega1

    Plasmid

    #165860

    Purpose

    Omega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PdnaK-IR2 GFP pZa

    Plasmid

    #170081

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PdnaK-IR2

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLyGo-Bs-4

    Plasmid

    #163142

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLyGo-Bs-3

    Plasmid

    #163141

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLyGo-Bs-2

    Plasmid

    #163140

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. IBMc180

    Plasmid

    #161942

    Purpose

    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK528

    Plasmid

    #155388

    Purpose

    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK434

    Plasmid

    #155378

    Purpose

    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFA-DAB1

    Plasmid

    #162691

    Purpose

    Expresses DabAB1 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB1 inorganic carbon transport complex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw

    Plasmid

    #162473

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreAB
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw

    Plasmid

    #162478

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreAB
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw

    Plasmid

    #162476

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreB
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw

    Plasmid

    #162475

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw

    Plasmid

    #162472

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreC
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw

    Plasmid

    #162470

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw

    Plasmid

    #162477

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreC
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw

    Plasmid

    #162471

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreB
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSWD95Y14D

    Plasmid

    #159098

    Purpose

    CckA FRET Sensor with cyclic-di-GMP point mutant

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutated Y514 to D514

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSWD95D293A

    Plasmid

    #159099

    Purpose

    CckA FRET Sensor with DITE motif mutant

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CckA D293 mutated to A293 and V199I on mRuby3 (P…

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDGE669

    Plasmid

    #153235

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SMT215

    Plasmid

    #134818

    Purpose

    SMT205 with DHFR-FLAG-tag, western blot

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SMT101

    Plasmid

    #134815

    Purpose

    Dual expression of DHFR and TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDGE672

    Plasmid

    #153238

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDGE473

    Plasmid

    #153264

    Purpose

    M1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDGE500

    Plasmid

    #153273

    Purpose

    M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDGE502

    Plasmid

    #153275

    Purpose

    M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. C335

    Plasmid

    #141125

    Purpose

    Expresses chimeric repressor (RbsR-L) under Cin promoter

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Inserts

    LLhR
    AiiA (AQ980_RS12585 Synthetic)
    

    Use

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Chimera: DNA binding domain of LacI and ligand bi…

    Promoter

    engineered cin promoter (responds to C14-HSL) and…

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. SMT201

    Plasmid

    #134816

    Purpose

    SMT101 with TET promoter for TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCASP-SptP35-TD-HilA

    Plasmid

    #153332

    Purpose

    Triggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    HA4-7c12 Tandem Monobody
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCASP-SptP92-TD-HilA

    Plasmid

    #153331

    Purpose

    Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP92-HA4-7c12 Tandem Monobody
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCASP-3G124-HilA

    Plasmid

    #153324

    Purpose

    Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    3G124 DARPin but no expression
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGBW-m4046774

    Plasmid

    #145574

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. pGBW-m4046846

    Plasmid

    #145575

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. MYMH 351

    Plasmid

    #138058

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-10AG 11AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAA GAG GAG GCA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge