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We narrowed to 10,563 results for: CHL

Showing: 9951 - 10000 of 10563 results

  2. pMpGWB122

    Plasmid
    #68576
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68576-plasmid-map-sequence-id-121831

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  3. VanGlow-RR

    Plasmid
    #83343
    Purpose
    for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    mCherry::Renilla(nls)
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83343-plasmid-map-sequence-id-158672

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  5. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  6. pSR284

    Plasmid
    #125093
    Purpose
    Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143
    Depositor
    Jeffrey Tabor
    Article
    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d
    Inserts
    ompR(REC)-manR(DBD)122
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    125093-plasmid-map-sequence-id-247832

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  8. pTSlb-wt

    Plasmid
    #60728
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60728-plasmid-map-sequence-id-99370

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  9. pNSSCPG

    Plasmid
    #183187
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183187-plasmid-map-sequence-id-366657

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  10. pSR241

    Plasmid
    #125091
    Purpose
    Expression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181
    Depositor
    Jeffrey Tabor
    Article
    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d
    Inserts
    ompR(REC)-yxdJ(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    125091-plasmid-map-sequence-id-247829

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  12. pHS1068

    Plasmid
    #205962
    Purpose
    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    DinG HNH proteins and CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac (proteins), pJ23119 (crRNA)
    Available since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    205962-plasmid-map-sequence-id-421621

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  13. N-BLa 1.1

    Plasmid
    #88994
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.1 fusion protein
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88994-plasmid-map-sequence-id-177496

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  18. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  19. pEasyG3_nat

    Plasmid
    #184918
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184918-plasmid-map-sequence-id-368408

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  20. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  22. pQZ210

    Plasmid
    #106414
    Purpose
    Co-expresses trans SNARE complex with pDC183 in E.coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Fragment 191-256 , Codon optimization and Fragmen…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106414-plasmid-map-sequence-id-204167

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  25. pPbuCas13b-gRNA-NT

    Plasmid
    #184568
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184568-plasmid-map-sequence-id-374001

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  26. pKD279

    Plasmid
    #125099
    Purpose
    Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Jeffrey Tabor
    Article
    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d
    Inserts
    SO_4388(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    125099-plasmid-map-sequence-id-245241

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  30. pMpGWB119

    Plasmid
    #68573
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68573-plasmid-map-sequence-id-121814

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  32. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

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  33. pRB113.2

    Plasmid
    #181948
    Purpose
    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181948-plasmid-map-sequence-id-356574

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  34. pMpGWB121

    Plasmid
    #68575
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    MpEF1alpha promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68575-plasmid-map-sequence-id-121822

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  35. pMuLE Lenti Dest IFP1.4

    Plasmid
    #62177
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Lentiviral; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62177-plasmid-map-sequence-id-347594

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  36. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid
    #127637
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    YPet
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PP7 coat protein (PCP)
    Expression
    Bacterial
    Mutation
    Promoter
    pJ21306
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127637-plasmid-map-sequence-id-251705

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  37. pEPQD1CB0107

    Plasmid
    #177040
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    177040-plasmid-map-sequence-id-349013

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  38. pMuLE Lenti Dest Luc2

    Plasmid
    #62179
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses firefly luciferase under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Luciferase; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62179-plasmid-map-sequence-id-101463

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  41. pMpGWB222

    Plasmid
    #68613
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68613-plasmid-map-sequence-id-121832

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  42. pMpGWB219

    Plasmid
    #68610
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68610-plasmid-map-sequence-id-121815

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  44. R4pGWB401+Citrine

    Plasmid
    #128432
    Purpose
    Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.
    Depositor
    George Haughn
    Article
    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Citrine, yellow fluorescence protein
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    128432-plasmid-map-sequence-id-257650

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  45. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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  46. AEB-No-aptamer-controlluciferase

    Plasmid
    #63853
    Purpose
    This pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.
    Depositor
    Howard Salis
    Article
    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv
    Insert
    No-aptamer control mRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tac
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    63853-plasmid-map-sequence-id-106576

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  47. pMpGWB221

    Plasmid
    #68612
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    MpEF1alpha promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68612-plasmid-map-sequence-id-121823

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  48. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

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  50. pKD265

    Plasmid
    #125095
    Purpose
    Expression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Jeffrey Tabor
    Article
    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d
    Inserts
    torR(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    125095-plasmid-map-sequence-id-247895

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Showing: 9951 - 10000 of 10563 results