We narrowed to 15,768 results for: LEA

1. pET28a MISTIC

   Plasmid

   #85999

   Purpose

   bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   N/A
   

   Tags

   HIS6 and MISTIC

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX_HA

   Plasmid

   #178534

   Purpose

   Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-mPCSK7

   Plasmid

   #122676

   Purpose

   Expresses mouse PCSK7 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-NES-KIRIN1

   Plasmid

   #107416

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Tags

   Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

   Expression

   Mammalian

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-mNINJ1-GFP

   Plasmid

   #208776

   Purpose

   MSCV vector expressing WT mouse NINJ1

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   NINJ1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Available Since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Expression

   Bacterial

   Promoter

   prab17

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH-MBP-CbAgo

   Plasmid

   #127707

   Purpose

   pET His6 MBP TEV CbAgo

   Depositor

   John van der Oost

   Article

   Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

   Insert

   CbAgo
   

   Tags

   MPB

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-FLAG-PGRMC2

   Plasmid

   #173468

   Purpose

   Human PGRMC2 containing an N-terminal FLAG tag

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC2 (PGRMC2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

   Plasmid

   #228721

   Purpose

   Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2.2

    Plasmid

    #34885

    Depositor

    Irvin Chen

    Article

    Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

    Insert

    Sindbis virus envelope protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    SGN UTR in E1 domain

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHBV-Luc

    Plasmid

    #71414

    Purpose

    To see the activity of HBV transcription using Luciferase assay

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

    Insert

    HBV core promoter, enhancer I, enhancer II and luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    HBV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK305

    Plasmid

    #66812

    Purpose

    IVT vector for Fluc mRNA

    Depositor

    Ron Weiss

    Article

    Andries et al J Control Release. 2015 Sep 2. pii: S016

    Insert

    Firefly luciferase
    

    Use

    Luciferase; Mrna ivt

    Promoter

    T7

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-BE1

    Plasmid

    #73019

    Purpose

    Expresses BE1 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/V5-His Snk/Plk2

    Plasmid

    #16015

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

15. p15a_AcCas12n

    Plasmid

    #203806

    Purpose

    p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12n
    

    Use

    CRISPR

    Promoter

    rpsl

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PV-NES-GFP

    Plasmid

    #17301

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-NES-GFP (Pvalb Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2HA-pDEST-N

    Plasmid

    #118373

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Available Since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

    Plasmid

    #228733

    Purpose

    Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid eNme-C nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS/ES-EnvA-VSVg-WPRE-pA

    Plasmid

    #87221

    Purpose

    to package wild type EnvA psudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    EnvA-VSVg
    

    Use

    Mouse Targeting and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CT752

    Plasmid

    #233658

    Purpose

    Flp GFP Reporter; FRT-STOP-FRT-GFP

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    FRT-STOP-FRT-GFP
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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