4,143 results

1. OCT4-eGFP-PGK-Puro

   Plasmid

   #31937

   Purpose

   Oct4 targeting donor vector

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CaMKIIa-ChETA-EYFP

   Plasmid

   #26967

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T

   Promoter

   CaMKIIa

   Available since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-HA-Sox2-IP

   Plasmid

   #13459

   Depositor

   Shinya Yamanaka

   Article

   Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA cyclin D1 HA T286A

   Plasmid

   #11182

   Depositor

   Bruce Zetter

   Article

   Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

   Insert

   cyclin D1 T286A (CCND1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T286A. Mutant that cannot be degraded via the u…

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-tetO-lox-hKLF4

   Plasmid

   #20727

   Depositor

   Rudolf Jaenisch

   Article

   Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAL149

   Plasmid

   #22275

   Purpose

   Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB(1-908) (PHYB Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mCherry-Kras4BCT (prenylation sequence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACAGW-ChR2-Venus-AAV

   Plasmid

   #20071

   Purpose

   AAV-mediated expression of ChR2-Venus for neuronal excitation

   Depositor

   Karel Svoboda

   Article

   Leopoldo et al Nature. 2009 Jan 18.

   Has Service

   AAV1 and AAV9

   Insert

   Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)
   

   Use

   AAV

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. miR-146a

   Plasmid

   #15092

   Depositor

   David Baltimore

   Article

   Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   pre-miR-146a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RNT1-GFP

    Plasmid

    #17708

    Depositor

    Hal Dietz

    Article

    Mendell et al Science. 2002 Oct 11. 298(5592):419-22.

    Insert

    rent1 (UPF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS302

    Plasmid

    #11925

    Depositor

    Brian Sauer

    Article

    Sauer et al Methods Enzymol. 1993;225:890-900. doi:

    Insert

    STOP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1318 pcDNA3 flag FKHRL1

    Plasmid

    #10708

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHRL1 (FOXO3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pARF6(Q67L)-CFP

    Plasmid

    #11387

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC93-pmax-dCas9VP160

    Plasmid

    #48225

    Purpose

    dCas9VP160 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.3_mCherry

    Plasmid

    #26823

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-mCherry-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCMV5 Flag-Smad1

    Plasmid

    #14044

    Depositor

    Joan Massague

    Article

    Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

    Insert

    Smad1 (SMAD1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPRIME-CMV-GFP-FF3

    Plasmid

    #11663

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-Sall4

    Plasmid

    #13361

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    sal-like 4 (Drosophila) (Sall4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag Snail 6SA

    Plasmid

    #16221

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S96A, S100A, S107A, S111A, S115A, S119A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge