4,235 results

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pEZY3

Plasmid

#18672

Purpose

pcDNA3 converted Gateway Destination vector for mammalian expression

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pAC152-dual-dCas9VP64-sgExpression

Plasmid

#48238

Purpose

Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA Tag, HA-Tag, and VP64

Expression

Mammalian

Mutation

D10A;H840A

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-E2F4

Plasmid

#10914

Depositor

Bob Weinberg

Article

Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

Insert

E2F4 (E2F4 Human)

Expression

Mammalian

Available Since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only
V51 pIRESpuro-GLUE (pGLUE)

Plasmid

#15100

Purpose

tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-NES

Plasmid

#36206

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

NES

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pX330-Cetn1/1

Plasmid

#50718

Purpose

pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Inserts

Cetn1 sgRNA1
humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and hU6

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-P35

Plasmid

#1347

Depositor

Li-Huei Tsai

Insert

P35 (CDK5R1 Human)

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pY002 (pFnCpf1_min)

Plasmid

#69975

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes nd artificial promoter

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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LeGO-iT2

Plasmid

#27343

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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Cdk2-DN-HA

Plasmid

#1885

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

Plasmid

#50942

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Has Service

AAV1

Insert

mRuby2-P2A-GCaMP6s

Use

AAV

Promoter

hSyn1

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eNOS-GFP

Plasmid

#22444

Depositor

William Sessa

Article

Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

Insert

WT eNOS-GFP

Tags

GFP

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pABCb10-GFP

Plasmid

#19031

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

ATP-binding cassette, sub-family B (MDR/TAP), member 10 (Abcb10 Mouse)

Tags

HIS

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pN1 Lck-GCaMP5G

Plasmid

#34924

Purpose

Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

Depositor

Baljit Khakh

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

Lck-GCaMP5G

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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PGKneoF2L2DTA

Plasmid

#13445

Depositor

Philippe Soriano

Article

Hoch et al Development. 2006 Feb . 133(4):663-73.

Insert

PGKneoF2L2DTA

Use

Cre/Lox

Expression

Mammalian

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hOct4

Plasmid

#19778

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Oct4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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Cdk5-DN-HA

Plasmid

#1873

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Tags

HA

Expression

Mammalian

Mutation

D145N, dominant negative

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-puro

Plasmid

#51240

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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