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pRK5-HA GST RagB 99L

Plasmid

#19303

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q99L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pFLPo

Plasmid

#13792

Purpose

Expression of mouse codon-optimized FLP recombinase

Depositor

Philippe Soriano

Article

Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

Insert

FLPo

Use

Cloning vector

Expression

Bacterial

Available Since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

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Cas9m4-VP64

Plasmid

#47319

Purpose

Cas9m4 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m4-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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myc mTOR kinase dead

Plasmid

#8482

Depositor

David Sabatini

Insert

mTOR (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

D2357E V2364I

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad2

Plasmid

#14042

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Article

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pMSP1E2

Plasmid

#20063

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E2 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5-K130R

Plasmid

#13096

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5-K130R (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

K130R

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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paavCAG-Jx-rev-post-mGRASP-2A-dTomato

Plasmid

#34913

Purpose

JxON-post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

postsynaptic mGRASP

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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3544 pMIG Bcl-2

Plasmid

#8793

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-2 (BCL2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pGFP-HBx

Plasmid

#24931

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

EGFP and HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Article

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagB 99L

Plasmid

#19315

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q99L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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809 pcDNA3 GFP PTEN

Plasmid

#10759

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-GLIS1

Plasmid

#30166

Purpose

Retroviral expression of non-canonical human GLIS1

Depositor

Shinya Yamanaka

Article

Maekawa et al Nature. 2011 . 474(7350):225-9.

Insert

GLIS1 (GLIS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 10, 2011

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

Plasmid

#47868

Purpose

This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a and U6

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pWZL Blast DN E-cadherin

Plasmid

#18800

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

Dom neg E-cadherin (Cdh1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

truncated form of E-cadherin (dominant negative) …

Available Since

July 15, 2008

Availability

Academic Institutions and Nonprofits only

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HBT-pcoCas9

Plasmid

#52254

Purpose

transient expression of pcoCas9 gene in plant cells

Depositor

Jen Sheen

Article

Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

Insert

pcoCas9

Use

CRISPR; Plant expression

Tags

FLAG and NLS

Promoter

hybrid constitutive promoter 35SPPDK

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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FCK-ChR2-GFP

Plasmid

#15814

Purpose

Lentiviral expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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