We narrowed to 18,119 results for: URE

Showing: 6001 - 6030 of 18119 results

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zCREST3:Cas9green; EGF_gRNA

Plasmid

#199333

Purpose

pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTetR193-Wasabi

Plasmid

#86105

Purpose

mWasabi reporter gene gated by temperature-sensitive TetR I193N

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TetRts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

I193N

Promoter

ptetA and ptetR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G04

Plasmid

#67138

Purpose

Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

EGFP and HIS

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFH2.21_mCherry-MS2x6

Plasmid

#205536

Purpose

Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pVH233-Tier1-PmPGK1-eYFP

Plasmid

#169522

Purpose

Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven enhanced yellow fluorescent protein

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt9b

Plasmid

#42287

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt9b (Wnt9b Mouse)

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
pCIS.MTRIA-NPY

Plasmid

#184613

Purpose

For expression of a fluorescent sensor for neuropeptide Y in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NPY

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-SST

Plasmid

#184619

Purpose

For expression of a fluorescent sensor for somatostatin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-SST

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP2A13

Plasmid

#67103

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2A13 (CYP2A13 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMS17: pTwinStrep-SUMO-AvTnsD

Plasmid

#168150

Purpose

AvTnsD expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsD

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

Plasmid

#116963

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFH2.11_Gag-MCP_ZF2-P2A-eGFP

Plasmid

#205526

Purpose

Express HIV Gag-MCP_ZF2-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP_ZF2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 RAD54B

Plasmid

#67124

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RAD54B (RAD54B Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pHCMV_LUHF_GPC

Plasmid

#207290

Purpose

Mammalian expression of Lujo virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

LUHF_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_MACV_GPC

Plasmid

#207291

Purpose

Mammalian expression of Machupo virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MACV_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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py100-sgRNA_hVEGFA

Plasmid

#198282

Purpose

Expression of SpCas9 guide RNA specific for human VEGFA locus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

py100-sgRNA_hVEGFA

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Cer.zf1

Plasmid

#61389

Purpose

mRNA synthesis of cerulean codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Cer.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-TMED3

Plasmid

#136014

Purpose

TMED3 3'UTR (NM_007364.3) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

TMED3 3'UTR (TMED3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-Pnf

Plasmid

#198321

Purpose

For affinity purification of Pnf payload

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

TwinStrep-SUMO-Pnf

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-MoonTag

Plasmid

#198299

Purpose

Produces PVCs retargeted with MoonTag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-MoonTag

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJOG824

Plasmid

#105401

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

SV40 nuclear localization signal

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

March 27, 2018

Availability

Academic Institutions and Nonprofits only

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1.6 kB VEGF-luc

Plasmid

#29667

Depositor

Patricia D'Amore

Article

Shima et al J Biol Chem. 1996 Feb 16. 271(7):3877-83

Insert

1.6 kB VEGF

Use

Luciferase

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET23a-LIR-1 D1-D2

Plasmid

#12103

Depositor

Pamela Bjorkman

Article

Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

Insert

Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)

Expression

Bacterial

Mutation

Amino acids 1−198*

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
EGFP-Rab33bb in pCSDEST2

Plasmid

#140882

Purpose

Expression of zebrafish GFP-tagged Rab33bb in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab33bb

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2

Plasmid

#145370

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Tags

His and mCherry

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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