We narrowed to 4,995 results for: mcs

1. pMuLE (R4-R3) PRB

   Plasmid

   #206164

   Purpose

   pMuLE R4-R3 entry plasmid containing human full length PRB gene, for 3-component Gateway assembly

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   Progesteron receptor B (PGR Human)
   

   Use

   Gateway cloning vector

   Promoter

   -

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGoldenGreenGate-M (pGGG-M)

   Plasmid

   #165422

   Purpose

   A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Level 2 golden gate (moclo) du…

   Promoter

   Contain GG cassette and MCS with LacZ for blue/wh…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pM123: pAAV-EFS-CasRx-control presgRNA

   Plasmid

   #166871

   Purpose

   AAV vector for expressing CasRx and control presgRNA for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-control presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.155_LV-Puro-T2A-mTagBFP2

   Plasmid

   #205553

   Purpose

   Express puromycin resistance marker and mTagBFP2

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Puro-T2A-mTagBFP2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-DeltaFosB

   Plasmid

   #68545

   Purpose

   AAV expression of DeltaFosB

   Depositor

   Eric Nestler

   Article

   Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

   Insert

   DeltaFosB-IRES-GFP
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   truncated splice variant of FosB

   Promoter

   CMV

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pTol2-nbt-mTagBFP2-P2A-mScarlet

   Plasmid

   #196115

   Purpose

   zebrafish expression of nbt-mTagBFP2-P2A-mScarlet

   Depositor

   Reinhard Köster

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   nbt-mTagBFP2-P2A-mScarlet
   

   Use

   Zebrafish expression

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMMP-dnPERK-IRES-eGFP

   Plasmid

   #36954

   Depositor

   Bill Sugden

   Article

   Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

   Insert

   dnPERK (EIF2AK3 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   deletion of aa592-1061 to create dominant negative

   Promoter

   CMV

   Available Since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH_Str-KDEL_neomycin

   Plasmid

   #65307

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
   

   Use

   Lentiviral

   Tags

   KDEL motif and Signal Sequence

   Promoter

   CMV

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFDH

   Plasmid

   #131706

   Purpose

   Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

   Depositor

   Ron Milo

   Article

   Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

   Insert

   formate dehydrogenase
   

   Tags

   his-tag

   Expression

   Bacterial

   Available Since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI H2B-GFP

    Plasmid

    #92399

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-SypHer2-DAAO-NES

    Plasmid

    #119184

    Purpose

    Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

    Depositor

    Thomas Michel

    Article

    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

    Insert

    SypHer2-DAAO-NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2-nbt-Electra2-P2A-mScarlet

    Plasmid

    #196114

    Purpose

    zebrafish expression of nbt-Electra2-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-Electra2-P2A-mScarlet
    

    Use

    Zebrafish expression

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH_SBP-EGFP-Ecadherin

    Plasmid

    #65290

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-TetO-tdTomato-cNLS

    Plasmid

    #138532

    Purpose

    Lentiviral inducible expression of tdTomato with nuclear localization signal

    Depositor

    Ana Teixeira, Christopher Grigsby

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-GfaABC1D-iCreV-4x6T

    Plasmid

    #196412

    Purpose

    Astrocytic expression of light-inducible iCreV recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    iCreV
    

    Use

    AAV; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH_SBP-mCherry-GPI

    Plasmid

    #65299

    Purpose

    synchronize trafficking of mCherry-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS4134

    Plasmid

    #131747

    Purpose

    Human reduced folate transporter 1 SLC19A1 for lentiviral transduction, copGFP transduction marker

    Depositor

    Christina Smolke

    Article

    Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

    Insert

    Human reduced folate carrier 1 (SLC19A1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV3Tag8li-hSAPS1-HA

    Plasmid

    #165466

    Purpose

    Human SAPS1 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Protein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUW-TetO-mCherry-cNLS

    Plasmid

    #138531

    Purpose

    Lentiviral inducible expression of mCherry with nuclear localization signal

    Depositor

    Ana Teixeira, Christopher Grigsby

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH_SBP-mCherry-Ecadherin

    Plasmid

    #65291

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge