164,577 results

1. pX330A-1x6

   Plasmid

   #58770

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 Dendra2

   Plasmid

   #103967

   Purpose

   Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

   Depositor

   Qing Deng

   Article

   Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

   Insert

   Dendra2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330S-2

   Plasmid

   #58778

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVH1002-Tier2(pUC ori)

   Plasmid

   #169600

   Purpose

   Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET20b_Pa-PirA

   Plasmid

   #128956

   Purpose

   expresses Pseudomonas aeruginosa PirA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
   

   Use

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR-L5-Kir2.1-mCherry-L2

   Plasmid

   #32669

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   Kir2.1-mCherry (Kcnj2 Mouse)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBintE

   Plasmid

   #48136

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUCDM

   Plasmid

   #110742

   Purpose

   Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin and p10 promoters

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV14_NHP2L1(15.5kD)

   Plasmid

   #73065

   Purpose

   expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   NHP2L1 (15.5kD) (SNU13 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_AGIA-AirID

    Plasmid

    #182210

    Purpose

    Express AirID in mammalian cells.

    Depositor

    Akira Nozawa, Sohei Ito, Shogo Nakano, Tatsuya Sawasaki

    Article

    Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

    Insert

    AGIA-AirID
    

    Use

    Tags

    AGIA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHIV-Zsgreen

    Plasmid

    #18121

    Depositor

    Zena Werb, Bryan Welm

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Insert

    Zsgreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPB_EF1a_Gag-EIF4E

    Plasmid

    #212657

    Purpose

    piggyBac vector to express an MLV Gag-EIF4E fusion protein

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Piggybac

    Tags

    EIF4E

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCG_IFITM2

    Plasmid

    #179956

    Purpose

    Expression vector for human IFITM2.

    Depositor

    Konstantin Sparrer, Frank Kirchhoff

    Article

    Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

    Insert

    IFITM2 (IFITM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A33V, T41M, V121I

    Promoter

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P-rTetO-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170325

    Purpose

    Tetracycline-inducible expression of V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    Eric Lander, David Sabatini

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHO-UAS-mScarlet-I

    Plasmid

    #183147

    Purpose

    Yeat two-hybrid fluorecent reporter for genomic integration

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-SP-FRB (T2098L) -sfGFP-TM-V5

    Plasmid

    #186227

    Purpose

    mRNA synthesis of rapalog-induced cell adhesion molecule

    Depositor

    Yoshihiro Morishita

    Article

    Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

    Insert

    mRNA synthesis of rapalog-induced cell adhesion molecule
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    FRB (T2098L)

    Promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACT-Lex

    Plasmid

    #183149

    Purpose

    Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 AD
    

    Use

    Yeat two-hybrid

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sec61 beta pAcGFP-C1 (802)

    Plasmid

    #62008

    Purpose

    mammalian expression of GFP-Sec61 beta

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only