We narrowed to 10,740 results for: AGA

1. EGFP-p300 (D1399Y)

   Plasmid

   #191763

   Purpose

   Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   FRB-EGFP-p300 core (D1399Y) (EP300 Human)
   

   Use

   Tags

   EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

   Expression

   Bacterial and Mammalian

   Mutation

   catalytic D1399Y mutation in p300

   Promoter

   EF1alpha

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234445

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNTK human MBD3 PGK-Neo-pA flox

   Plasmid

   #52362

   Purpose

   Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   MBD3 (MBD3 Human)
   

   Use

   Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

   Plasmid

   #132667

   Purpose

   Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-ODD-T2A-TdT (DNTT Human, Streptococcus pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   4xHRE_YB TATA

   Available since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234446

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 Sox2 3H b

   Plasmid

   #26352

   Depositor

   Matthew Meyerson

   Article

   Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

   Insert

   Sox2 shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

7. lucMAPT-30D

   Plasmid

   #214672

   Purpose

   MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site

   Depositor

   Eugene Yeo

   Article

   Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   Firefly Luciferase, Renilla Luciferase, and V5

   Expression

   Mammalian

   Mutation

   Contains Exon 9, Exon 10 with a mutation to intro…

   Promoter

   CMV

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. lucMAPT-30U

   Plasmid

   #214673

   Purpose

   MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site

   Depositor

   Eugene Yeo

   Article

   Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   Firefly Luciferase, Renilla Luciferase, and V5

   Expression

   Mammalian

   Mutation

   Contains Exon 9, Exon 10 with a mutation to intro…

   Promoter

   CMV

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCXIP-mCherry-Halo-YAP1

   Plasmid

   #128336

   Purpose

   Retrovirus construct to express mCherry-Halo-YAP1

   Depositor

   Yutaka Hata

   Article

   Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry and Halo-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAGI1 gRNA (BRDN0001487062)

    Plasmid

    #78068

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAGI1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS puro HA-hUSP27X

    Plasmid

    #225718

    Purpose

    Bacterial expression of USP27X with HA tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. A3Bi-ctd-Cas9n-UGI-NLS

    Plasmid

    #109426

    Purpose

    Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AIP(WT)-mChF-giantin

    Plasmid

    #61523

    Purpose

    Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Inserts

    FK506 binding protein 1A (FKBP1A Human)
    golgin B1 (GOLGB1 Human)
    

    Use

    Tags

    AIP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pRSFDuet-FBXL5-SKP1

    Plasmid

    #226620

    Purpose

    Co-expression of FBXL5-SKP1 complex in E.coli

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Inserts

    FBXL5 (FBXL5 Human)
    SKP1 (SKP1 Human)
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    Amino acids 1-198 was deleted, and 420-596 was re…

    Promoter

    T7

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-NanoLuc-BRAF-CAT

    Plasmid

    #194772

    Purpose

    Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
    

    Use

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-BRAF-REG-Halo

    Plasmid

    #194771

    Purpose

    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpp1-is2

    Plasmid

    #58248

    Purpose

    Expression vector producing isoform 2 derived mouse osteopontin (EGFP tagged)

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Spp1-is2 (Spp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mRFP-FKBP12-5ptpase domain

    Plasmid

    #67516

    Purpose

    Recruitable human type IV 5-phosphatase domain (214-644)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mutation

    INPP5E has C641A to destroy the C-terminal CAAX d…

    Promoter

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpp1-is3

    Plasmid

    #58247

    Purpose

    Expression vector producing isoform 3 derived mouse osteopontin (EGFP tagged)

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Spp1 isoform 3 (Spp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-CMV_ACE2-Flag_Hygro

    Plasmid

    #191998

    Purpose

    Lentiviral vector to generate flag-tagged ACE2 stable expressing cell line

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MAPK1 gRNA (BRDN0001147355)

    Plasmid

    #77787

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO.1 mTagBFP2 rat PGK1 shRNA

    Plasmid

    #222869

    Purpose

    Expresses mTagBFP2 along with an shRNA against rat PGK1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1 shRNA (Pgk1, Pgk1 Rat, Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-CMV-TMPRSS2-TwinStrep_Zeo

    Plasmid

    #192000

    Purpose

    Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Tags

    TMPRSS2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FUW-Flag-Mbd3 delta1-70

    Plasmid

    #52372

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-70

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FUW-Flag-Mbd3 delta 25-83

    Plasmid

    #52373

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    amino acids 25-83 deleted

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mRFP-FKBP12-5ptpase enzyme

    Plasmid

    #67515

    Purpose

    Recruitable human type IV 5-phosphatase enzyme

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    INPP5E has C641A to destroy the C-terminal CAAX d…

    Promoter

    CMV

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

28. FUW-Flag-Mbd3 delta49-63

    Plasmid

    #52374

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 49-63

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP.mIKKβ

    Plasmid

    #74412

    Purpose

    Expresses mouse eGFP-IKKβ in mammalian cells

    Depositor

    Georgios Stathopoulos

    Article

    Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

    Insert

    mouse IKKβ (Ikbkb Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MAP2K4 gRNA (BRDN0001145028)

    Plasmid

    #76650

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP2K4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP2K4 (MAP2K4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge