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pFB-HTB-ASK1

Plasmid

#29517

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

ASK1 (SKP1 Mustard Weed)

Expression

Insect

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pSLC-241

Plasmid

#73194

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-mqsR (mqsR )

Expression

Bacterial

Promoter

PrhaB

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFB-GTE-COI1

Plasmid

#29516

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

COI1 (COI1 Mustard Weed)

Tags

GST and TEV cleavage site

Expression

Insect

Mutation

Note that the first AA in TEV site is R, not E

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6A CARD8 CARD-FLAG WT

Plasmid

#164020

Purpose

Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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mRuby2-PMP-N-10

Plasmid

#55910

Purpose

Localization: Peroxisomes, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

PMP (PXMP2 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdKatushka2-VASP-5

Plasmid

#56049

Purpose

Localization: Actin/Focal Adhesions, Excitation: 586, Emission: 632

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

VASP

Tags

tdKatushka2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Syn-FLEX-ExSYTE

Plasmid

#216263

Purpose

Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE P2A EGFP - Rat DTE

Use

AAV

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GFP

Plasmid

#41614

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAM4937 (pCV0049)

Plasmid

#120082

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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rat Kv3.1-HA

Plasmid

#113055

Purpose

Expresses rat Kv3.1 in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

rat Kv3.1 (Kcnc1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Mutation

L1 intron insertion

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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Clover-pBAD

Plasmid

#54575

Purpose

Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and Clover

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
PLKO.1-CAPRIN1-3'UTR

Plasmid

#136055

Purpose

CAPRIN1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCACGTTAGTGTCACAAATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

CAPRIN1 (CAPRIN1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)

Plasmid

#105201

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-P2A-CCL21-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-3HA

Plasmid

#39283

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX303 GFP-PIK3C2A

Plasmid

#162000

Purpose

Lentiviral expression vector containing PIK3C2A tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Human)

Use

Lentiviral

Tags

eGFP

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_703128

Plasmid

#224394

Purpose

For expression of the type I CBASS system encoded by Escherichia coli 703128

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnD_4TM

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_BWH59

Plasmid

#224393

Purpose

For expression of the type I CBASS system encoded by Escherichia coli BWH59

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

2TM_EcCdnD

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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CFP-FRBx5-His

Plasmid

#103784

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

CFP-FRBx5-His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-TetR-KRf-TetR

Plasmid

#216270

Purpose

Construct 3: TetR - Lipid binding domain KRφ - TetR

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

TetR - Lipid binding motif (KRf) - TetR

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Caveolin-C-10

Plasmid

#55279

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Caveolin (CAV1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
YFP-FKBPx5

Plasmid

#103775

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP2(1)

Plasmid

#136060

Purpose

G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Expression

Mammalian

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-FKBPx5-His

Plasmid

#103785

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5-His

Tags

EYFP, His-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

Plasmid

#197952

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag Jip2

Plasmid

#59027

Purpose

act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

Depositor

Roger Davis

Article

Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

Insert

mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 )

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
mEmerald-H2B-C-10

Plasmid

#54112

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

Insert

H2B (H2BC11 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
px552-sg-gria1-HT-SEP

Plasmid

#187653

Purpose

Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

HaloTag-SEP donor

Use

AAV and CRISPR

Tags

N/A

Promoter

N/A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJG85

Plasmid

#89281

Purpose

Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMM9-MAPKK2-WT

Plasmid

#40805

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Tags

HA

Expression

Mammalian

Mutation

Wild type

Promoter

MSV-LTR

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only