We narrowed to 13,583 results for: TIM

1. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

   Plasmid

   #216377

   Purpose

   Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

   Depositor

   William Weis

   Insert

   LDL receptor related protein 6 (LRP6 Human)
   

   Use

   Baculovirus

   Tags

   9xHis and HA signal sequence-FLAG-3C cleavage sit…

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin (LRP6)/p10 (MESD)

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

   Plasmid

   #124603

   Purpose

   Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Has Service

   AAV9

   Insert

   Soma-localized ChrimsonR
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMLS716

   Plasmid

   #188888

   Purpose

   Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   floxed Cbr-unc-119 marker and Phsp16.41::Cre
   

   Use

   Tags

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-link-ytdTomato-URA3

   Plasmid

   #168054

   Purpose

   Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Use

   Tags

   ytdTomato

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXWJ109Hg-gfp

   Plasmid

   #123148

   Purpose

   A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, PmerT

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Integrin beta1(1-455)-tev-BHCys

   Plasmid

   #27315

   Depositor

   Timothy Springer

   Article

   Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

   Insert

   integrin beta1 (ITGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

   Promoter

   Available since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pSR49

   Plasmid

   #69259

   Purpose

   phlh-8::tagBFP2

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   TagBFP2
   

   Use

   Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0, TagBFP2 contains 2 sing…

   Promoter

   hlh-8

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MX01

   Plasmid

   #158624

   Purpose

   GATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).

   Depositor

   Timm Maier

   Article

   Scaiola et al Sci Adv. 2020 Nov 6;6(45). pii: 6/45/eab

   Type

   Empty backbone

   Use

   Gateway destination vector ; bacmam acceptor vect…

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MMLV-CAG-SADB19_oG-IRES-Puro

   Plasmid

   #172367

   Purpose

   Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   oG + Puro
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C1-Lyn-mCitrine-EEVEE-LifeAct

    Plasmid

    #155226

    Purpose

    Alternate MPAct using Lyn/LifeAct instead of CaaX/F-tractin

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+_Flag-myc-NES-Tir1

    Plasmid

    #117717

    Purpose

    mRNA synthesis of Flag-myc-NES-TIR1 for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    Flag-Myc-NES

    Expression

    Mammalian

    Mutation

    codon optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PIP2-CFP(ST) sensor

    Plasmid

    #170355

    Purpose

    CFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

    Depositor

    Kees Jalink

    Article

    van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

    Insert

    CFP(ST)-PH (PLCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Gi-c001

    Plasmid

    #173078

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5_tAKARα

    Plasmid

    #119913

    Purpose

    Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Accessory plasmid 2

    Plasmid

    #128571

    Purpose

    for cloning identified individual SPECS

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAP24

    Plasmid

    #70190

    Purpose

    Modular vector for secreted luciferase reporter expression

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

    Insert

    sLucopt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. plRL118

    Plasmid

    #163632

    Purpose

    Renilla luciferase reporter plasmid; Giles integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mycobacterial Optimized Promoter (MOP)

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p415TEF cyto roGFP2-Grx1(yeast)

    Plasmid

    #65004

    Purpose

    Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

    Insert

    Glutaredoxin-1
    

    Use

    Tags

    roGFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pc3xTC20-alphaTubulin

    Plasmid

    #36327

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

    Insert

    alpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling (TUBA1B Human, Synthetic)
    

    Use

    Tags

    3xTC20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENO1-iRFP-NATr

    Plasmid

    #129731

    Purpose

    Integration plasmid to overexpress iRFP in Candida albicans

    Depositor

    Robert Wheeler

    Article

    Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

    Insert

    iRFP-Candida albicans codon-optimized
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1 from Candida albicans

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAC-CR7T-gRNA2.1-nlsBFP

    Plasmid

    #170515

    Purpose

    gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CR7T

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GG-EBNA-OCT4

    Plasmid

    #69537

    Purpose

    Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    5 concatenated gRNA transcriptional cassettes targeting OCT4
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. UMG-SP-1_pET29b

    Plasmid

    #215433

    Purpose

    Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. UMG-SP-2_pET29b

    Plasmid

    #215434

    Purpose

    Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBEAST_J23101-amidase

    Plasmid

    #128135

    Purpose

    The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    amidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti6.3-efSaCas9-BlastR

    Plasmid

    #220971

    Purpose

    Expresses human codon-optimized efSaCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    efSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Use

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMB22

    Plasmid

    #122244

    Purpose

    Lentivirus delivery for stable expression of AsCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Expression

    Mutation

    Promoter

    EFs

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCEP4-DU422gp160

    Plasmid

    #100926

    Purpose

    Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (DU422.1) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NylB_pET29b

    Plasmid

    #215414

    Purpose

    Expression construct for NylB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. JH03

    Plasmid

    #73512

    Purpose

    mKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mKate2::PH::tbb-2
    

    Use

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    mex-5

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p119 (FUGW-UbCp-rtTA3)

    Plasmid

    #105171

    Purpose

    ubiquitin C promoter drives rtTA3 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    UbCp-rtTA3
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCEP4-B41gp160

    Plasmid

    #123247

    Purpose

    Mammalian expression plasmid for Env from the B41 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (B41) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSR40

    Plasmid

    #69257

    Purpose

    plin-31::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    lin-31

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTU1-A-SP44-mVenus-I

    Plasmid

    #163763

    Purpose

    Expresses Streptomyces codon-optimised mVenus-I

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    mVenus-I
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    SP44

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSCL.028

    Plasmid

    #184965

    Purpose

    Test effect of extending -Eco1 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJFRC7-UAS-NPRR_ANP

    Plasmid

    #133243

    Purpose

    Fluorescent reporter for neuropeptide release imaging

    Depositor

    David Anderson

    Article

    Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

    Insert

    rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1 FLAG-SIV-RCM-vpx

    Plasmid

    #115844

    Purpose

    Encodes codon optimized FLAG tagged SIVRCM-Vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM-Vpx
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pETCON-PTX-S1-220

    Plasmid

    #67747

    Purpose

    Pertussis Toxin Subunit 1 Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    PTxS1-220
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pNC1125

    Plasmid

    #41561

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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45. pSBY3_ligD-HygR

    Plasmid

    #104625

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. JH04

    Plasmid

    #73513

    Purpose

    mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mRuby2::PH::tbb-2
    

    Use

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    mex-5

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pYPQ290

    Plasmid

    #129670

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    AacCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCEP4-CH111gp160

    Plasmid

    #123268

    Purpose

    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

    Plasmid

    #31477

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 T2A mTagBFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    F13Y + S111Y on I-AniI = Y2 "reo" (res…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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50. mCas13 Twin-SUMO

    Plasmid

    #199753

    Purpose

    Expresses codon optimized miniature mCas13 for protein expression in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

    Insert

    mCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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