We narrowed to 14,039 results for: TIM

1. PB_EF1a-CasRx-msfGFP-2A-Blast

   Plasmid

   #226010

   Purpose

   CasRx protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   CasRx RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

   Plasmid

   #62459

   Purpose

   expresses his-tagged C288S human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Changed Cysteine 288 of Keap1 to serine

   Promoter

   CMV

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-4mtD3cpv

   Plasmid

   #181874

   Purpose

   Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   4mtD3cpv
   

   Use

   AAV and Adenoviral

   Expression

   Mammalian

   Mutation

   the 4mtD3cpv CDS differs from the recorded GeneBa…

   Promoter

   CaMKIIa

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYZ145

   Plasmid

   #98405

   Purpose

   Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Inserts

   upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
   downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. hCCR5-LSSmOrange

   Plasmid

   #110194

   Purpose

   Encodes for human CCR5 coding sequence tagged with the LSS-mOrange FP

   Depositor

   Sergi Padilla Parra

   Article

   Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

   Insert

   hCCR5 (CCR5 Human)
   

   Tags

   LSSmOrange

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYZ149

   Plasmid

   #98407

   Purpose

   Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Inserts

   upstream homologous region to delete his3 in genome by recombination (alg2 Fission Yeast)
   downstream homologous region to delete his3 in genome by recombination (cog8 Fission Yeast)
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Intron-Tagging-pX330-Cas9-mCherry

   Plasmid

   #159742

   Purpose

   Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

   Plasmid

   #154829

   Purpose

   Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   coEPO
   coEPO
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   Codon-optimized (Cricetulus griseus)

   Promoter

   100RPU.2

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p35u4

   Plasmid

   #174786

   Purpose

   Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system

   Depositor

   Katherine Berry

   Article

   Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126

   Insert

   MS2 coat protein
   

   Tags

   λCI

   Expression

   Bacterial

   Mutation

   V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

   Promoter

   Synthetic Constitutive Promoter

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRB117.2

    Plasmid

    #181949

    Purpose

    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 narL - E. coli, Synthetic)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 FRT TO myc hSeparase

    Plasmid

    #59820

    Purpose

    Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLdCH

    Plasmid

    #84291

    Purpose

    Express gRNA and Cas9 in Leishmania with Hygromycin resistance

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

    Insert

    gRNA and Cas9
    

    Use

    CRISPR; Leishmania

    Promoter

    L. donovani ribosome RNA promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

    Plasmid

    #134333

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA17_Efa
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19.TRIM28.FKBP.knock-in.mRuby2

    Plasmid

    #185008

    Purpose

    For knocking in FKBP F36V P2A mRuby2

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    FKBP F36V P2A mRuby2
    

    Use

    Cloning

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPHR-iRFP713

    Plasmid

    #103818

    Purpose

    Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-iRFP713 (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH4169

    Plasmid

    #200108

    Purpose

    Prig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    snb-1 (snb-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prig-3

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. OmEF1aCas9P2ApuroSB

    Plasmid

    #165484

    Purpose

    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

    Depositor

    Dietmar Kueltz

    Article

    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

    Insert

    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    

    Use

    CRISPR; Transposon

    Promoter

    Oreochromis mossambicus EF1 alpha promoter (OmEF1…

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB1alpha2_SulR

    Plasmid

    #186425

    Purpose

    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-FOXO3a TM delta CT

    Plasmid

    #1797

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, delta CT (aa 468-673)

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

20. MMRN1-bio-His

    Plasmid

    #53409

    Purpose

    Expresses full-length Multimerin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    MMRN1 (MMRN1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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