We narrowed to 17,814 results for: multi
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Plasmid#15883DepositorInsertbeta2-microglobulin (B2m Mouse)
Available SinceJan. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus
Plasmid#105127PurposeUsed along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent proteinDepositorInsertsUseZebrafish transgenesisPromotercryaa (crystallin) and fabp10aAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP
Plasmid#75148PurposeTagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
TagsEGFPPromoter5UASAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2 DN-GSK3-GFP
Plasmid#29681DepositorInsertglycogen synthase kinase 3
TagsGFPExpressionMammalianMutationK85R and K86RAvailable SinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBABE-MCS(extended)-Puro (empty vector)
Plasmid#172603PurposeEmpty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianMutationThe multiple cloning site (MCS) has been edited/e…Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK277 (mClover-NeoR)
Plasmid#72793PurposeC-terminal tagging with mClover using CRIPSR/Cas9DepositorInsertmClover-Neo
UseCRISPRTagsmCloverExpressionMammalianAvailable SinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGM1202
Plasmid#69615Purposeinducible gene expression in Streptomyces, C-terminal His-tag encoding sequenceDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
Available SinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-gRNA
Plasmid#158973PurposeA human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.DepositorInsertgRNA3
ExpressionMammalianPromoterU6Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134B2.0
Plasmid#167158PurposeGolden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyExpressionMammalianPromoterEf1alphaAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-HaloTag9
Plasmid#175543PurposeExpression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cellsDepositorInsertLyn11-HaloTag9
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyto-YFP-FKBPx5
Plasmid#103777Purposeexpression of iPOLYMER component in mammalian cells; has nuclear exclusion sequenceDepositorInsertcyto-YFP-FKBPx5
ExpressionMammalianPromoterCMVAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA(MS2)-puro-barcode
Plasmid#89493PurposeCloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seqDepositorInsertsgRNA (with MS2 loop)
UseLentiviralPromoterU6Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nanog-2A-mCherry
Plasmid#48680PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCMV-AcGFP
Plasmid#131008PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianPromoterpCMV and pTRE3G-BiAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3cH_pGAP_23*_pLAT1_Cas9
Plasmid#104907PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only