We narrowed to 9,077 results for: APP

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pmCellFree_KA2_ZIKV NS3

Plasmid

#145365

Purpose

Cell-free/mammalian expression vector for Zika virus NS3 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Availability

Academic Institutions and Nonprofits only

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pEMY01AB-PRO

Plasmid

#127714

Purpose

Integrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pEMY01CD-TER

Plasmid

#127751

Purpose

Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pC3.1_CAG_oROS-Gr

Plasmid

#216113

Purpose

Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

CAG_oROS-Gr

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLX303-ZIM3-KRAB-dCas9

Plasmid

#154472

Purpose

Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSBinit

Plasmid

#110100

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

myc, His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b_PstS_1

Plasmid

#78198

Purpose

Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A197C)

Expression

Bacterial

Mutation

A197C (please see depositors comment below)

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMD2-VSVGmut

Plasmid

#182229

Purpose

Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

VSVGmut

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CCR5

Plasmid

#98943

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pKB01_mCherry

Plasmid

#69104

Purpose

Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2

Plasmid

#25031

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP2 (DCP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfpC

Plasmid

#111616

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSUM-GroEL-gfp

Plasmid

#109286

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GroEL-GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

GroEL

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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hSyn-Cre-hSyn-PreRNA barcode landing pad (PPIB)

Plasmid

#247718

Purpose

The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

Cre and PreRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-Cre-hSyn-PostRNA barcode landing pad (dapB)

Plasmid

#247720

Purpose

The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

Cre and PostRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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APX1-H2B-mEos3.2

Plasmid

#245522

Purpose

Plasmid for PiggyBac /ITR mediated insertion of H2B-mEos sequence downstream of CAG promoter using Gateway recombination cassette

Depositor

Timothy Sanders, Kate Storey

Article

Dady et al Development. 2025 Aug 1:dev.205082. doi:

Insert

H2B-mEos

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Has Service

Cloning Grade DNA

Insert

cas9

Use

CRISPR; Lambda red recombinase

Expression

Bacterial

Promoter

native cas9 promoter

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLR16-Phes*

Plasmid

#98783

Purpose

Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

Depositor

Anat Herskovits

Article

Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

Insert

pheS

Expression

Bacterial

Mutation

A309G

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b_PstS_2

Plasmid

#78199

Purpose

Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A17C,A197C)

Expression

Bacterial

Mutation

A17C; A197C (please see depositor comment below)

Promoter

T7

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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