We narrowed to 7,509 results for: PAC;

1. pTPH405c

   Plasmid

   #185726

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-N-Flag-8His

   Plasmid

   #157677

   Purpose

   N-terminal tagging vector

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Flag-8His
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA5-FRT-TO-eGFP

   Plasmid

   #157733

   Purpose

   mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG223 PRKD1 3'UTR mut

   Plasmid

   #12042

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   PRKD1 3'UTR mut (PRKD1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Promoter

   Available since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

5. p2T-CMV-evoCjCas9-BE4max-BlastR

   Plasmid

   #194061

   Purpose

   Mammalian expression of evoCjCas9 CBE4max

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM552

   Plasmid

   #154131

   Purpose

   Expresses wt rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   wt bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-P3-evoCjCas9-TadCDd_gRNA

   Plasmid

   #202563

   Purpose

   P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-TadCDd
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. dSp-VP64-p65(100-261)-RTA(125-190)

   Plasmid

   #99673

   Purpose

   Expresses dSp Cas9 fused to combination of truncated activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   VP64-p65(101-261)-RTA(125-190)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-dSa-VPR mini.

   Plasmid

   #99686

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVITRO-HPV31 L1L2

    Plasmid

    #52600

    Purpose

    Expresses HPV31 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV31 L1
    HPV31 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVITRO-HPV35 L1L2

    Plasmid

    #52492

    Purpose

    Expresses HPV35 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV35 L1
    HPV35 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-dSa-VPR mini.-2X snRP1

    Plasmid

    #99688

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

    Plasmid

    #72304

    Purpose

    Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

    Depositor

    Adam Cohen

    Article

    Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

    Insert

    CaViar
    

    Use

    Lentiviral

    Tags

    TS

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL-SaBE3

    Plasmid

    #85169

    Purpose

    Expresses SaBE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaBE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-mF(3230)A3

    Plasmid

    #168016

    Purpose

    Expresses MBP-F(3230)A3-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-F(3230)A3-6xHis
    

    Use

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ133-RZ-Cas12j2

    Plasmid

    #189784

    Purpose

    Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-P3-evoCjCas9-eAID_gRNA

    Plasmid

    #202562

    Purpose

    P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-eAID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSB1K3_M13ps_LacZα_gIII

    Plasmid

    #190435

    Purpose

    M13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    M13 packaging signal
    M13 gIII
    LacZα
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge