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ABL1_HUMAN_D0

Plasmid

#79727

Purpose

This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

ABL1 (ABL1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-UbC-tdTomato

Plasmid

#62516

Purpose

Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Insert

tdTomato

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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Flag Leucyl tRNA synthetase pRK5

Plasmid

#46341

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

leucyl tRNA synthetase (LARS1 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-KPTN

Plasmid

#87041

Purpose

mammalian expression of KPTN

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

KPTN (KPTN Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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mScarlet-MYO10

Plasmid

#145179

Purpose

Expresses mScarlet-MYO10 in mammalian cells

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

mScarlet-MYO10 (MYO10 Human)

Expression

Mammalian

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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SaABEmax

Plasmid

#119814

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSaCas9

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pmSTARRseq1

Plasmid

#96945

Purpose

CpG free plasmid for testing effects of methylation on regulatory activity

Depositor

Jenny Tung

Type

Empty backbone

Use

Mstarr-seq screening vector

Expression

Mammalian

Promoter

CpG -free EF1 promoter

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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LeGO-G

Plasmid

#27347

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pTag-RFP-C-h-Rab5a-c-Myc

Plasmid

#79802

Purpose

Express TagRFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Tags

TagRFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pS-cr:GFP

Plasmid

#196295

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs

Use

CRISPR

Tags

SpeI-DR-BsaI-DR-SpeI

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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psd44-iGFP-MFGE8-long

Plasmid

#46847

Purpose

expression of MFGE8 in mammalian cells

Depositor

Agnese Mariotti

Article

Carrascosa et al Oncogene. 2012 Mar 22;31(12):1521-32. do

Insert

MFGE8 (Mfge8 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK promoter

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only
pCR-blunt-TOPO-FNDC5

Plasmid

#35970

Depositor

Bruce Spiegelman

Article

Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

Insert

fibronectin type III domain containing 5 (Fndc5 Mouse)

Use

Cloning vector

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

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CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pnlacZ-ACN

Plasmid

#20304

Depositor

Mario Capecchi

Article

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Insert

nlacZ-ACN

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

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spNW100

Plasmid

#173488

Purpose

Expresses spNW100 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW100

Tags

Histag

Expression

Bacterial

Mutation

P1049L- please see depositor comments

Promoter

T7

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBAC-RT

Plasmid

#16418

Depositor

Craig Strathdee

Article

Stavropoulos et al Genomics. 2001 Feb 15. 72(1):99-104.

Insert

E. coli rpsL gene; tetA(C) gene

Use

Bacmid

Available Since

June 2, 2008

Availability

Academic Institutions and Nonprofits only
pLenti_CamKIIa_mKate2

Plasmid

#96942

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

far-red fluorescent protein

Use

Lentiviral

Mutation

E87D (please see depositor comments below)

Promoter

CaMKIIa

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-Itch/2793

Plasmid

#11434

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

Itch (Itch Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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LeGO-iCer

Plasmid

#27363

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, IRES-Cerulean

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pSEMS-CV SUy-Halo7Tag

Plasmid

#108933

Purpose

Selflabeling tag at ATP Synthase

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

ATP synthase F1 subunit gamma (ATP5F1C Human)

Tags

Halo7 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-N2-PKCgamma

Plasmid

#21204

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKC gamma (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

PKC gamma (rat). (Lab plasmid WC 0013)

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-CFP-STM2(15-746)

Plasmid

#18863

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal Interaction Molecule 2 (STIM2 Human)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between signal peptide and residues …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

Plasmid

#126705

Purpose

donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

GFP

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MED4

Plasmid

#15425

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED4 (MED4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F uGFP

Plasmid

#14558

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174) in DH domain

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
AAV9retro

Plasmid

#224687

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

Zhigang He

Article

Zhang et al Cell. 2024 May 3:S0092-8674(24)00447-1.

Insert

Rep2/Cap9retro

Use

AAV

Mutation

insertion of 10-mer peptide from AAV2retro (LADQD…

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209725

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209730

Purpose

Expresses presynaptic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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3265 pBS Mcl-1

Plasmid

#8763

Depositor

Stanley Korsmeyer

Article

Rinkenberger et al Genes Dev 2000 Jan 1;14(1):23-7.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Cloning vector

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only