We narrowed to 5,494 results for: SPI

1. CAG_IGF1R_EGFP

   Plasmid

   #199628

   Purpose

   FRET donor for IGF1R sensor

   Depositor

   Ryohei Yasuda

   Article

   Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

   Insert

   IGF1R (IGF1R Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmTurqoise KIF1A shRNA

   Plasmid

   #102849

   Purpose

   Generates shRNA specific to Rat KIF1A along with cell fill pmTurqoise

   Depositor

   Erik Dent

   Article

   McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

   Insert

   KIF1A-shRNA (KIF1A Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1-RNA

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD_sfGFP3TCG4TAG

   Plasmid

   #174518

   Purpose

   Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   sfGFP
   

   Use

   Tags

   6 x His

   Expression

   Bacterial

   Mutation

   Recoded. TCG and TAG codons were introduced in po…

   Promoter

   araBAD

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF-G1(4CNW)RS

   Plasmid

   #222871

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-MyosinVa-Ctail

   Plasmid

   #110169

   Purpose

   Dominant negative effect by competing with MyosinVa C-terminal tail interactions

   Depositor

   Jose Esteban

   Article

   Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

   Insert

   Myosin Va (Myo5a Mouse)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   amino acids 1444-1853

   Promoter

   CMV

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLO 62.4

   Plasmid

   #123123

   Purpose

   Human expression vector for deltaproteobacteria CasX and sgRNA

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBLO 62.5

   Plasmid

   #123124

   Purpose

   Human expression vector for planctomyces CasX and sgRNA

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG_mIGF1R_EGFP

   Plasmid

   #199629

   Purpose

   mutant FRET donor for IGF1R sensor

   Depositor

   Ryohei Yasuda

   Article

   Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

   Insert

   mIGF1R (IGF1R Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Y1316F

   Promoter

   CAG

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172316

   Purpose

   CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-LbCpf1-Blast

    Plasmid

    #84751

    Purpose

    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    LbCpf1
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-3xHA (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

    Plasmid

    #194222

    Purpose

    Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    SynNotch-GAL4 DBD-VP64 AD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

    Plasmid

    #48795

    Purpose

    expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Inserts

    YWHAE (YWHAE Human)
    YWHAZ (YWHAZ Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (IRES)

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF-G1(6CNW)RS

    Plasmid

    #222873

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-UBC-hAAT-IRES-ZsGreen-W

    Plasmid

    #126702

    Purpose

    lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

    Depositor

    Darrell Kotton

    Article

    Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

    Insert

    Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UBC

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SARS_COV2_ORF9B_HA_BASU

    Plasmid

    #153981

    Purpose

    Express Orf9b gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    orf9b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAd-Track Flag-SIRT1 H355A

    Plasmid

    #8439

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Histidine 355 mutated to Alanine

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ230-RRVQ

    Plasmid

    #200188

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V, E795Q

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST_DSUP_RAMVA (pBS0353)

    Plasmid

    #185189

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD_Ub11TCA

    Plasmid

    #174517

    Purpose

    Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    Ubiquitin
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Recoded with TCA replacing codon 11 of wt ubiquit…

    Promoter

    araBAD

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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