We narrowed to 5,328 results for: pcDNA3.1

1. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

   Plasmid

   #169548

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-BaLgp140-8his

   Plasmid

   #100924

   Purpose

   Mammalian expression plasmid for soluble gp140 from the BaL HIV-1 strain

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BaL) Env
   

   Tags

   CD5 leader sequence and Purification tag after D6…

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

   Plasmid

   #159283

   Purpose

   A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Human INS sgRNA
   Human INS guide RNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CREB3L3

   Plasmid

   #99509

   Purpose

   to express CREB3L3 in mammalian system

   Depositor

   Dong-Yan Jin

   Article

   Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

   Insert

   CREB3L3 (Creb3l3 Mouse)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTracer_Cr-TRP1

   Plasmid

   #64881

   Purpose

   Expresses CrTRP1 in mammalian cells

   Depositor

   Sebastián Brauchi

   Article

   Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

   Insert

   Transient receptor potential 1
   

   Tags

   eGFP

   Promoter

   CMV

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. miniABEMax V82G wt

   Plasmid

   #135362

   Purpose

   Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax nSpyCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaMKIV-dCT

   Plasmid

   #126422

   Purpose

   Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

   Depositor

   Douglas Black

   Article

   Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

   Insert

   Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PH Akt-Venus

   Plasmid

   #85223

   Purpose

   Venus tagged PH domain of Akt for use as a PIP3 sensor

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

   Insert

   PH AKT(1-148) (AKT1 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ALIX(1-702)-mNeonGreen

   Plasmid

   #191193

   Purpose

   Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

   Depositor

   Jin Zhang

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX (1-702) (PDCD6IP Human, Synthetic)
   

   Tags

   6xHIS and mNeonGreen

   Expression

   Mammalian

   Mutation

   Deletion of ALIX C-terminal PRD.

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3_altPKA-Cα-mEGFP

    Plasmid

    #114132

    Purpose

    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    18747 (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA flag-Msi1

    Plasmid

    #184029

    Purpose

    Msi1 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Msi1 (Msi1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA_SZ1-RGG-GFP-RGG

    Plasmid

    #124932

    Purpose

    Expression of SZ1-RGG-GFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Vh-HA-3xFKBP

    Plasmid

    #20222

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFKBP (FKBP1A Human)
    

    Tags

    HA and Vh

    Expression

    Mammalian

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

14. DN-LOXL2

    Plasmid

    #200062

    Purpose

    LOXL2 deletion mutant lacking 1-338 amino acids

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    N-terminus amino acids 1-338 truncated.

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. R338G/V339P-LOXL2

    Plasmid

    #200063

    Purpose

    Point mutant of hLOXL2 resistant to processing by Factor Xa

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    S300P mutation

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mSIRT3-S

    Plasmid

    #33310

    Depositor

    Michael Sack

    Article

    Bao et al J Cell Biochem. 2010 May;110(1):238-47.

    Insert

    Sirt3 (Sirt3 Mouse)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA SF-HERC2 F1

    Plasmid

    #55784

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
    

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Vh-HA-FKBP

    Plasmid

    #20220

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FKBP (FKBP1A Human)
    

    Tags

    HA and Vh

    Expression

    Mammalian

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-ING4

    Plasmid

    #13289

    Depositor

    Curtis Harris

    Article

    Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

    Insert

    ING4 (ING4 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only