We narrowed to 7,654 results for: pac

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pAAV-CMV-dSa-VPR mini.-syn pA

Plasmid

#99689

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP

Plasmid

#157733

Purpose

mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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BPK3082

Plasmid

#78742

Purpose

Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

LbCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-ABE8e_gRNA

Plasmid

#202559

Purpose

P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A

Plasmid

#102611

Purpose

Control lentivector expressing Puro resistance and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAB1619 hU6-BpiI-Cas13bt1-DR

Plasmid

#176314

Purpose

hU6-BpiI-Cas13bt1-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt1 crRNA direct repeat

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV11 L1L2

Plasmid

#52590

Purpose

Expresses HPV11 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV11 L1
HPV11 L2

Expression

Mammalian

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pC020 - LseCas13a from Listeria seeligeri

Plasmid

#91910

Purpose

Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LseCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-TadCDd_gRNA

Plasmid

#202563

Purpose

P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

AAV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-eAID_gRNA

Plasmid

#202562

Purpose

P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

AAV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMP31-1

Plasmid

#45905

Depositor

Harmit Malik

Article

Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

Insert

mitochondrial antiviral signaling protein (MAVS Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_wt_Venus_wt

Plasmid

#39813

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx3

Plasmid

#202753

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

Plasmid

#72304

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2-sgControl

Plasmid

#230080

Purpose

Crispr knock out control

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

non-specific sequence control

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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Marathon-RT pentamutant for Split-PE (pEH107)

Plasmid

#190110

Purpose

Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Tags

bpNLS

Expression

Mammalian

Mutation

D14R, N26R, D74R, N116K, N197R

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BE1

Plasmid

#73019

Purpose

Expresses BE1 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-SacB

Plasmid

#113635

Purpose

Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

Depositor

Miguel Valvano

Article

Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

Insert

sacB

Expression

Bacterial

Mutation

Please see Depositor Comments

Promoter

sacB promoter

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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