We narrowed to 13,642 results for: NTS

1. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

   Plasmid

   #192400

   Purpose

   On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-Rluc-B3RT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

   Plasmid

   #192402

   Purpose

   On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28 basic.2

   Plasmid

   #191911

   Purpose

   Encodes a GFP design, basic.2

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   basic.2
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72A V112I Y145V L220V V224I

   Promoter

   T7

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

   Plasmid

   #192405

   Purpose

   Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   B3RT-Act2-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

   Plasmid

   #192403

   Purpose

   Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   FRT-Act2-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

   Plasmid

   #192381

   Purpose

   To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

   Plasmid

   #192379

   Purpose

   To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

   Plasmid

   #192377

   Purpose

   To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

   Plasmid

   #192378

   Purpose

   To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-MBP-CI2F

    Plasmid

    #191254

    Purpose

    Expresses chymotrypsin inhibitor with an N-terminal MBP fusion protein

    Depositor

    Arthur Laganowsky

    Article

    Raab et al J Am Chem Soc. 2020 Oct 14;142(41):17372

    Insert

    Chymotrypsin Inhibitor 2
    

    Use

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDR111_mannose_Bgal NLS

    Plasmid

    #188398

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 2Bc-T MBP_BoMoC(ed)_W403A_6xH

    Plasmid

    #185712

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1C-Fluc-par6_D

    Plasmid

    #146117

    Purpose

    Insect Expression of par6-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

    Insert

    par6-3UTR (par-6 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmMe31B-mut1_E

    Plasmid

    #146203

    Purpose

    Insect Expression of DmMe31B-mut1

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-mut1 (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAT15488_HeF-ABE8e

    Plasmid

    #174126

    Purpose

    plasmid expressing HeF-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HeF-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAT15487_evo-ABE8e

    Plasmid

    #174125

    Purpose

    plasmid expressing evo-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evo-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAT15486_HypaR661A-ABE8e

    Plasmid

    #174124

    Purpose

    plasmid expressing HypaR661A-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HypaR-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAT15484_HF-ABE8e

    Plasmid

    #174122

    Purpose

    Expresses ABE8e fused with SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HF-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6xHis-CPL2/pET28a

    Plasmid

    #172095

    Purpose

    cDNA of CER11/CPL2 cloned into pET28a for protein expression

    Depositor

    George Haughn

    Article

    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

    Insert

    CPL2 (CPL2 Mustard Weed)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. CPL2g/pDONR221

    Plasmid

    #172093

    Purpose

    Genomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221

    Depositor

    George Haughn

    Article

    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

    Insert

    CPL2 (CPL2 Mustard Weed)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    CPL2

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pLenti-3xFLAG-Puro PAK5 S573N

    Plasmid

    #167195

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 S573N

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 S573N
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCerulean3-13aa-Venus-pEGFP-C3

    Plasmid

    #166753

    Purpose

    For expression of mCerulean3 linked to Venus by 13 amino acid linker

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3-13aa-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCerulean3-39aa-Venus-pEGFP-C3

    Plasmid

    #166755

    Purpose

    For expression of mCerulean3 linked to Venus by 39 amino acid linker

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3-39aa-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMP71-mGFP-LLO-hPMEL

    Plasmid

    #174607

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    expresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAT9994-HypaR661A-ABE

    Plasmid

    #163002

    Purpose

    plasmid expressing HypaR661A-ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    R661A

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAT9996-HeF-ABE

    Plasmid

    #163004

    Purpose

    plasmid expressing HeF-ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HeF-ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19-whiteCopyCatcher

    Plasmid

    #174062

    Purpose

    CopyCatcher insertion donor for the white locus

    Depositor

    Ethan Bier

    Article

    Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

    Insert

    white (w Synthetic, Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJS697

    Plasmid

    #168778

    Purpose

    Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Type

    Empty backbone

    Use

    Tags

    Cmyc and HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. E1_pENTR_L1-L4_NI_G1333-DddtoxA-C

    Plasmid

    #171724

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. E1_pENTR_L1-L4_NN_G1333-DddtoxA-C

    Plasmid

    #171728

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of a cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge