We narrowed to 5,494 results for: SPI

1. pcDNA3.1-Hyg-mEGFP

   Plasmid

   #191847

   Purpose

   Expresses the monomeric form of EGFP

   Depositor

   Bertrand Collet

   Article

   Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SARS_COV2_orf9c_HA_BASU

   Plasmid

   #153991

   Purpose

   Express Orf9c gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf9c
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

   Plasmid

   #204288

   Purpose

   yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

   Depositor

   Linda Huang

   Article

   Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

   Insert

   pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   pHis3

   Available since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRetro-XT-PAK2-CFP(puro)-T402A

   Plasmid

   #81179

   Purpose

   Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

   Depositor

   Angeliki Malliri

   Article

   Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

   Insert

   p21 (RAC1) activated kinase 2 (PAK2 Human)
   

   Use

   Retroviral

   Tags

   CFP

   Expression

   Mutation

   T402A

   Promoter

   P-tight TRE

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PBLO CasX-gRNA

   Plasmid

   #126419

   Purpose

   E. coli vector for CasX gRNA --- Used for In Vitro Transcription

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   casX gRNA
   

   Use

   CRISPR; In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CoV-2-NSP3-c002

   Plasmid

   #173084

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E1084-E1192, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScalps ACE2 hygro

   Plasmid

   #184786

   Purpose

   Stable expression of Human Ace2

   Depositor

   Ajit Chande

   Article

   Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pY005 (pcDNA3.1-hLb3Cpf1)

   Plasmid

   #69977

   Purpose

   Expresses humanized Lb3Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb3Cpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pST_CAHS3_HYPDU (pBS0333)

   Plasmid

   #185179

   Purpose

   For the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS3_HYPDU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pST_APOE_HUMAN_E114I (pBS0839)

    Plasmid

    #185274

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_E114I
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E114I

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRetro-XT-PAK2-CFP(puro)-L107F

    Plasmid

    #81180

    Purpose

    Retroviral vector for expression of kinase active PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mutation

    L107F

    Promoter

    P-tight TRE

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

12. Arch(D95H)-eGFP

    Plasmid

    #51081

    Purpose

    Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

    Depositor

    Adam Cohen

    Article

    Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:

    Insert

    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Aspartic Acid 95 to Histidine

    Promoter

    Ubiquitin

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBP-GTSE1-His6

    Plasmid

    #69109

    Purpose

    For bacterial expression of recombinant human GTSE1 tagged with MBP and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    G2- and S-phase Expressed 1 (GTSE1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-7 asyn S129G

    Plasmid

    #36049

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129G (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S129G

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIP EGFP-ER [TM(SAC1)]

    Plasmid

    #186475

    Purpose

    Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    SAC1 (SACM1L Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-terminal transmembrane region of the protein: 5…

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST_AfrLEA3m (pBS0911)

    Plasmid

    #185285

    Purpose

    For the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    AfrLEA3m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST_PvLEA4_repeats_k3_26 (pBS0762)

    Plasmid

    #185244

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-IRES-GFP-Hybrid-caspase_human-caspase-4-CARD+canine-Caspase-1/4-catalytic domain

    Plasmid

    #183397

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    CARD domain of human caspase-4 fused to catalytic domain of canine caspase-1/4
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 7749 Bicistronic_ires_hygro

    Plasmid

    #64374

    Purpose

    This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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