We narrowed to 40,628 results for: ANT
-
Plasmid#218436Purposemammalian expression plasmid for the heavy chain fragment of an anti-vGAT Fab domainDepositorInsertvGAT-Vh-hIgG1-CH1
UseAffinity Reagent/ AntibodyTagsBM40 and TwinStrep-6xHisExpressionMammalianPromoterCMVAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9050
Plasmid#219429PurposePapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)DepositorInsertAATG_PapE-4xLF2_AGGT
UseMoclo compatible level 0 moduleAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ROR2-V5/HIS
Plasmid#205608PurposeExpression of human ROR2 receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet-I3-NCwt-SYFP2
Plasmid#189765PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet-I3-NCwt-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-mRFP-pcDNA3.1-
Plasmid#52510Purposeexpresses mRFP in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertmRFP
TagsFLAG-HAExpressionMammalianMutationengineered variant of tetrameric red fluorescent …PromoterCMVAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-HA-GFP11-KDEL
Plasmid#177722PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
TagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
SSTR3-DuET
Plasmid#213378PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPY1R-DuET
Plasmid#213354PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-PuroR
Plasmid#196962PurposeExpresses PE2 with puromycin resistanceDepositorInsertPE2-P2A-Puro
ExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-C12orf66
Plasmid#87048Purposemammalian expression of C12orf66DepositorAvailable SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
OPN3-DuET
Plasmid#213357PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO:Δluc::gfp ΔAmpR::KanR
Plasmid#176640PurposeMPRAu backbone vectorDepositorInsertemGFP
ExpressionMammalianPromoterHuman PGK-1Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
p403 – TDH3-MTS-mCherry-GFP1-10-His
Plasmid#177730PurposemCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS markerDepositorInsertmCherry-GFP1-10
TagsMitochondrial Targeting SequenceExpressionBacterial and YeastAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE32-pr-mEosFP (mEosFP-V69T)
Plasmid#99213PurposeBacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).DepositorInsertpr-mEosFP
ExpressionBacterialMutationmonomeric variant with mutation V69T for primed c…PromoterT5Available SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
OPRD1-DuET
Plasmid#213358PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ROS1-V5/HIS
Plasmid#205609PurposeExpression of human ROS1 receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
UTS2R-DuET
Plasmid#213386PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A1
Plasmid#135503PurposeMammalian expression of myc-tagged HLA-A*01:01DepositorInsertHLA-A*01:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-N90-beta-catenin
Plasmid#31787DepositorInsertbeta-catenin (CTNNB1 Human)
UseEntry vectorTagsMycMutationDeletion of aa 1-90- Constitutively ActiveAvailable SinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
Affibody HER2:243.BirA*-pET301/NT-DEST
Plasmid#174692PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-ITFG2
Plasmid#87045Purposemammalian expression of ITFG2DepositorAvailable SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
NTSR1-DuET
Plasmid#213356PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ErbB-3-pGL3
Plasmid#60899Purposehuman ErbB-3 promoter reporter constructDepositorAvailable SinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-Golgin-84-TEV #709#243
Plasmid#42108DepositorInsertGolgin-84 (GOLGA5 Human)
TagsGFPExpressionMammalianMutationinserted ENLYFQG b/w 300 and 301PromoterCMVAvailable SinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
P2RY11-DuET
Plasmid#213364PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLINTR mCHERRY v1 backbone
Plasmid#179778PurposeSPLINTR backbone vectorDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRa42_4454
Plasmid#49703DepositorInsertECF42_4454
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
SSTR2-DuET
Plasmid#213377PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TIE-V5/HIS
Plasmid#205612PurposeExpression of human TIE receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet
Plasmid#130708PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP
Plasmid#139485PurposePCR template for Halo-Tag gene knock-in donor vectorDepositorInsertHaloTag-loxP-P2A-dTKneo-pA-loxP
TagsHaloTagExpressionMammalianPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPLINTR GFP v1 backbone
Plasmid#179777PurposeSPLINTR backbone vectorDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B8
Plasmid#135512PurposeMammalian expression of myc-tagged HLA-B*08:01DepositorInsertHLA-B*08:01 (HLA-B Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S
Plasmid#62459Purposeexpresses his-tagged C288S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
TagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 288 of Keap1 to serinePromoterCMVAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd
Plasmid#135518PurposeMammalian expression of myc-tagged H2-DdDepositorInsertH2-Dd (H2-D1 Mouse)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationC121SPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE1
Plasmid#195367PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAGDepositorInserthA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten plus 3…Tags3xflagExpressionPlantPromoterZmUBI1Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
TagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationS135APromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
DN-LOXL2
Plasmid#200062PurposeLOXL2 deletion mutant lacking 1-338 amino acidsDepositorInserthuman lysyl oxidase like 2 (LOXL2 Human)
TagsHisExpressionMammalianMutationN-terminus amino acids 1-338 truncated.Available SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only