We narrowed to 5,494 results for: SPI

1. CKAP2-mNeonGreen pHAT

   Plasmid

   #175947

   Purpose

   Bacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagII

   Depositor

   Susanne Bechstedt

   Article

   McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

   Insert

   CKAP2 (Ckap2 Mouse)
   

   Use

   Tags

   6His, StrepTagII, and mNeonGreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST_SERF2_HUMAN (pBS0751)

   Plasmid

   #185239

   Purpose

   For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   SERF2_HUMAN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST_MAHS_RAMVA (pBS0310)

   Plasmid

   #185169

   Purpose

   For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   MAHS_RAMVA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTK165_Mem-mCherry-4.1G-CTD

   Plasmid

   #46362

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   4.1G C-terminal domain (EPB41L2 Human)
   

   Use

   Tags

   Mem (N-terminal 20 amino acids of neuromodulin fo…

   Expression

   Mammalian

   Mutation

   C-terminal domain from 886-1005 amino acids

   Promoter

   CMV

   Available since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-SARS-CoV-2 SL1 alone-Luciferase

   Plasmid

   #191482

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

   Depositor

   Hao Wu

   Article

   Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

   Insert

   SARS-CoV-2 5' UTR SL1 alone
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Contains only the stem-loop1 region of SARS-CoV-2…

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-dCpf1-BE0

   Plasmid

   #107678

   Purpose

   Expresses dLbCpf1-BE0 in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D832A/E925/D1148A

   Promoter

   CMV

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSicoR-Ef1a-mCh-GFPi

   Plasmid

   #31849

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DsRed +9e

   Plasmid

   #191766

   Purpose

   Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Use

   Tags

   N terminal fusion of positively charged polypetod…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pST_DHN_AtCor47 (pBS0784)

   Plasmid

   #185254

   Purpose

   For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DHN_AtCor47
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSicoR-Ef1a-mCh-Puro-Luci

    Plasmid

    #31850

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    Luciferase RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDK197

    Plasmid

    #134189

    Purpose

    Retroviral plasmid expressing LAP-Clasp C terminus

    Depositor

    Iain Cheeseman

    Article

    Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

    Insert

    LAP-Clasp C terminus (CLASP1 Human)
    

    Use

    Retroviral

    Tags

    LAP (GFP)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Nanog-CreER targeting construct

    Plasmid

    #59720

    Purpose

    Cre-ER knockin targeting construct for mouse endogenous Nanog locus

    Depositor

    Jacob Hanna

    Article

    Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

    Insert

    creERT2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    mNanog

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CKAP2 pHAT

    Plasmid

    #175946

    Purpose

    Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

    Depositor

    Susanne Bechstedt

    Article

    McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

    Insert

    CKAP2 (Ckap2 Mouse)
    

    Use

    Tags

    6-His and StrepTagII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHelA12

    Plasmid

    #176493

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

    Depositor

    Jian-Dong Huang, Xiaodong Cheng

    Article

    Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

    Insert

    SARS-CoV NSP13 (ORF1ab SARS-CoV)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    V570I

    Promoter

    T7

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Venus-G protein Gamma 5

    Plasmid

    #42200

    Depositor

    Narasimhan Gautam

    Article

    Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

    Insert

    Gamma 5 (Gng5 Rat)
    

    Use

    Tags

    Venus(1-155)

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

17. R9-WT

    Plasmid

    #125210

    Purpose

    Expressing wildtype human Rad9b gene

    Depositor

    Richard Finnell

    Article

    Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

    Insert

    Rad9b (RAD9B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 8200 Bicistronic_GFP_ires_hygro

    Plasmid

    #64375

    Purpose

    This a retroviral expression plasmid expressing GFP along with hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEMT-Tyrosinase-bait-T2a-Cherry-pA

    Plasmid

    #111155

    Purpose

    knock in bait for Axolotl Tyrosinase locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

    Insert

    Tyrosinase-bait-T2a-Cherry-pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

    Plasmid

    #172317

    Purpose

    CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge