We narrowed to 5,566 results for: SPI

1. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

   Plasmid

   #194222

   Purpose

   Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   SynNotch-GAL4 DBD-VP64 AD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAd-Track Flag-SIRT1 H355A

   Plasmid

   #8439

   Depositor

   Pere Puigserver

   Article

   Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Histidine 355 mutated to Alanine

   Promoter

   Available since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS_COV2_ORF9B_HA_BASU

   Plasmid

   #153981

   Purpose

   Express Orf9b gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf9b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF-G1(6CNW)RS

   Plasmid

   #222873

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CYFIP2.mCherry

   Plasmid

   #122052

   Purpose

   Expresses human CYFIP2 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP2 (CYFIP2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiviral shRNA GEF-H1

   Plasmid

   #21477

   Depositor

   Richard Huganir

   Article

   Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

   Insert

   rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5-FRT/TO-LacR-TopBP1

   Plasmid

   #31313

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

   Insert

   TopBP1 (TOPBP1 Human)
   

   Use

   Tags

   FLAG and LacR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pBAD_Ub11TCA

   Plasmid

   #174517

   Purpose

   Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   Ubiquitin
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Recoded with TCA replacing codon 11 of wt ubiquit…

   Promoter

   araBAD

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBLO ddd64.1

   Plasmid

   #123121

   Purpose

   E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

   Depositor

   Jennifer Doudna, Benjamin Oakes

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   CasX
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Mutated amino acids in catalytic triad to alanine…

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP64T-hLbCpf1

    Plasmid

    #107187

    Purpose

    plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mutation

    Promoter

    sp6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ230-RRVQ

    Plasmid

    #200188

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V, E795Q

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST_DSUP_RAMVA (pBS0353)

    Plasmid

    #185189

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

    Plasmid

    #89636

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEX-Antenna-GDI RhoA

    Plasmid

    #189669

    Purpose

    Expression of Antenna-RhoA

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ230-RVQ

    Plasmid

    #200187

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V, E795Q

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CB6-GFP-TAOK2 (K57A)

    Plasmid

    #197114

    Purpose

    Expression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

    Insert

    TAOK2 (K57A) (TAOK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed K 57 to A

    Promoter

    CMV

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SARS_COV2_ORF3b_HA_BASU

    Plasmid

    #153992

    Purpose

    Express Orf3b gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    orf3b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-EFS-FlpO

    Plasmid

    #191509

    Purpose

    Lentiviral Flp Recombinase driven by the EFS promoter

    Depositor

    Eric Snyder

    Article

    Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

    Insert

    flp recombinase (flp )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/EFS promoter inserted

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-mCherry2-sgMUC4

    Plasmid

    #198399

    Purpose

    Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    MUC4 sgRNA (MUC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.1-nsp7-FLAG

    Plasmid

    #201027

    Purpose

    Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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