We narrowed to 9,400 results for: Coli

1. His6_MBP_FXR1P

   Plasmid

   #198694

   Purpose

   Expression of human FXR1 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR1P (FXR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PARP9-c024

   Plasmid

   #173096

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   G102-K298

   Promoter

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET14-Encap

   Plasmid

   #86405

   Purpose

   Expresses T. maritima encapsulin in E. coli

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Insert

   Encapsulin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PARP9-c020

   Plasmid

   #173097

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N310-N493

   Promoter

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Parp14-c012

   Plasmid

   #173099

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   W1003-A1190

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENV8(WT)-GFPuv

   Plasmid

   #70127

   Purpose

   Cobalamin riboswitch reporter vector for use in E. coli

   Depositor

   Robert Batey

   Article

   Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

   Insert

   wild type environmental cobalamin riboswitch sequence (env8)
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Parp14-c010

   Plasmid

   #173100

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   F1208-G1388

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPIPL12-FnCas12a

   Plasmid

   #199563

   Purpose

   Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

   Depositor

   Philippe Lambin

   Article

   Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

   Insert

   Fncas12a
   

   Use

   CRISPR and Synthetic Biology; Tetracycline induci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   COtetR-PIPL12

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCJR01

   Plasmid

   #187888

   Purpose

   Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Insert

   DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PARP15-c020

    Plasmid

    #173101

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    N78-S267, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Dead-Laconic/pcDNA3.1(-)

    Plasmid

    #118627

    Purpose

    To express in mammalian cells a lactate FRET sensor negative control.

    Depositor

    Luis Felipe Barros

    Article

    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

    Insert

    Dead-Laconic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Change Histidine 151 to Aspartic Acid of Escheric…

    Promoter

    CMV Promoter

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-6xHis-GFP(+30)

    Plasmid

    #199164

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+30)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB1C3-Lux

    Plasmid

    #109383

    Purpose

    Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-LUX operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b-BE1

    Plasmid

    #73018

    Purpose

    Expresses BE1 with N-terminal His6 tag in E. Coli

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEVA221pmCDA1-T7RNAP

    Plasmid

    #167978

    Purpose

    Expresses fusion protein pmCDA1-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    pmCDA1-T7RNAP (T7p07 Synthetic)
    

    Use

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-P70a-3WJdB-T

    Plasmid

    #87311

    Purpose

    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Three-way Junction dimeric Broccoli aptamer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29b-pduCDE

    Plasmid

    #110138

    Purpose

    Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

    Insert

    pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPdX

    Plasmid

    #85084

    Purpose

    Expresses putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Cysteine 73 to Serine and Cysteine 85 to …

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCC25

    Plasmid

    #177319

    Purpose

    Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

    Depositor

    Rafal Tomecki

    Article

    Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

    Insert

    TtCutA 240-603
    

    Use

    Tags

    6xHis-SUMOTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. 3xFLAG-dCas9/p-bacteria

    Plasmid

    #64325

    Purpose

    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR; Tetracycline-inducible

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    pLtetO-1

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. His SUMO TEV SPOP 1-374

    Plasmid

    #197015

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    6His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTXRE5-Z

    Plasmid

    #112509

    Purpose

    Reporter plasmid, responsive to activated Ah receptor/Arnt

    Depositor

    Charles Miller

    Article

    Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

    Insert

    RW95-3 with 5x xenobiotic response elements (lacZ E.coli, Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    5 XREs+ minimal Cytochrome C

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3-mitoT

    Plasmid

    #174519

    Purpose

    Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

    Depositor

    Oleh Khalimonchuk

    Article

    Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

    Insert

    MitoT synthetic tether
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCherry-eDHFR

    Plasmid

    #107266

    Purpose

    mCherry-eDHFR in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    mCherry-eDHFR (dhfr E.coli)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-APOBEC1-YTH

    Plasmid

    #200158

    Purpose

    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTH
    

    Use

    Tags

    6xHis, HA, and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SurA

    Plasmid

    #213138

    Purpose

    Expresses chaperone SurA

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SurA (21-428) (surA E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His6_MBP_FXR2P_P474S_P493N

    Plasmid

    #198695

    Purpose

    Expression of human FXR2 protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FXR2P (FXR2 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Changed proline 474 to serine and proline 493 asp…

    Promoter

    T7

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge